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07-211 Anti-phospho-Met (Tyr1234/1235) Antibody

Detect phospho-Met (Tyr1234/1235) using this Anti-phospho-Met (Tyr1234/1235) Antibody validated for use in WB.

Anti-phospho-Met (Tyr1234/1235) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: MET, AUTS9, c-Met, RCCP2, HGFR UniProt Number: P08581
07-211
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-211
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Met (Tyr1234/1235) Antibody
Alternate Names
  • c-Met
  • cmet
  • HGFR
  • RCCP2
  • met proto-oncogene (hepatocyte growth factor receptor)
References
Product Information
FormatAffinity Purified
Control
  • NIH-3T3 cells co-expressing MET/HGFR and HGF
PresentationImmunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.1mM EDTA, 0.05% sodium azide before the addition of glycerol to 30%.
Applications
ApplicationDetect phospho-Met (Tyr1234/1235) using this Anti-phospho-Met (Tyr1234/1235) Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogenpeptide (DKE[pY][pY]SVHNK-C) corresponding to amino acids 1231-1240 of human phospho-Met/HGFR
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes phosphorylated Met.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.
Gene Symbol
  • MET
  • AUTS9
  • c-Met
  • RCCP2
  • HGFR
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08581 # Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity.
SIZE: 1390 amino acids; 155527 Da
SUBUNIT: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P08581 # Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. & Defects in MET may be associated with gastric cancer. & Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. & Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. & Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
SIMILARITY: SwissProt: P08581 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 3 IPT/TIG domains. & Contains 1 protein kinase domain. & Contains 1 Sema domain.
Molecular Weight140 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from NIH-3T3 cells co-expressing MET/HGFR and HGF
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-211 04053252728747

Documentation

Anti-phospho-Met (Tyr1234/1235) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-Met (Tyr1234/1235) - 20438 20438
Anti-phospho-Met (Tyr1234/1235) - 21837 21837
Anti-phospho-Met (Tyr1234/1235) - 23486 23486
Anti-phospho-Met (Tyr1234/1235) - 23746 23746
Anti-phospho-Met (Tyr1234/1235) - 25335 25335
Anti-phospho-Met (Tyr1234/1235) - 30022 30022

References

Reference overviewApplicationPub Med ID
In vitro differentiation of human adipose tissue-derived stem cells into cells with pancreatic phenotype by regenerating pancreas extract.
Jiyeon Lee,Duck-Jong Han,Song-Cheol Kim
Biochemical and biophysical research communications  375  2008

Show Abstract 		18725201 18725201
Synergistic effect of focal adhesion kinase overexpression and hepatocyte growth factor stimulation on cell transformation
Chan, P. C., et al
J Biol Chem, 277:50373-9 (2002)  2002

Immunoblotting (Western)12393896 12393896