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OP43 Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP43
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Fe, HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP43-100UG Plastic ampoule 100 μg
OP43-20UG Plastic ampoule 20 μg
Description
OverviewRecognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
Catalogue NumberOP43
Brand Family Calbiochem®
Application Data
Detection of human p53 by immunoblotting. Sample: Whole cell lysates from A431 cells. Primary antibody: Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) (Cat. No. OP43) (1 μg/ml). Detection: chemiluminescence.
References
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlSK-OV-3 cells or normal skin tissue
Positive controlA431 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoprecipitation and Immunocytochemistry Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Paraffin Sections, Frozen Sections, Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Immunoblotting Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Paraffin Sections
Application NotesFrozen sections (1 µg/ml; see application references)
Immunoblotting (0.1-1 µg/ml; see application references)
Immunocytochemistry (1-2.5 µg/ml; see application references)
Immunoprecipitation (1 µg/ml or use Cat. No. OP43A; see application references)
Paraffin sections (1 µg/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)
Application CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenwild type, recombinant, human p53
ImmunogenHuman
EpitopeWithin amino acids 21-25 of human p53
CloneDO-1
HostMouse
IsotypeIgG2a
Species Reactivity
  • Feline
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP43-100UG 04055977227543
OP43-20UG 04055977227123

Documentation

Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) Certificates of Analysis

TitleLot Number
OP43

References

Reference overview
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Fang Zhang, et al. (2006) Ribosomal stress couples the unfolded protein response to p53-dependent cell cycle arrest. Journal of Biological Chemistry 281, 30036-30045.
  • Frank Staib, et al. (2005) The p53 Tumor Suppressor Network is a Key Responder to Microenvironmental Components of Chronic Inflammatory Stress. Cancer Research 65, 10255-10264.
  • Hazel E. Warburton, et al. (2005) p53 regulation and function in renal cell carcinoma. Cancer Research 65, 6498-6503.
  • Urs Hobom and Matthias Dobbelstein. (2004) E1B-55-kilodalton protein is not required to block p53-induced transcription during adenovirus infection. Journal of Virology 78, 7685-7697.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision05-June-2008 JSW
    ApplicationFrozen sections (1 µg/ml; see application references)
    Immunoblotting (0.1-1 µg/ml; see application references)
    Immunocytochemistry (1-2.5 µg/ml; see application references)
    Immunoprecipitation (1 µg/ml or use Cat. No. OP43A; see application references)
    Paraffin sections (1 µg/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)
    Application Data
    Detection of human p53 by immunoblotting. Sample: Whole cell lysates from A431 cells. Primary antibody: Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) (Cat. No. OP43) (1 μg/ml). Detection: chemiluminescence.
    DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.
    BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death.
    HostMouse
    Immunogen speciesHuman
    Immunogenwild type, recombinant, human p53
    EpitopeWithin amino acids 21-25 of human p53
    CloneDO-1
    IsotypeIgG2a
    Speciesfeline, human, not mouse, not rat
    Positive controlA431 cells or breast carcinoma tissue
    Negative controlSK-OV-3 cells or normal skin tissue
    FormLiquid
    FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsRecognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 μl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
    Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
    Legros, Y., et al. 1994. Oncogene 9, 2071.
    Barak, Y., et al. 1993. EMBO J. 12, 461.
    Kastan, M.B., et al. 1992. Cell 71, 587.
    Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
    Lane, D.P. 1992. Nature 358, 15.
    Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
    Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.
    Citation
  • Fang Zhang, et al. (2006) Ribosomal stress couples the unfolded protein response to p53-dependent cell cycle arrest. Journal of Biological Chemistry 281, 30036-30045.
  • Frank Staib, et al. (2005) The p53 Tumor Suppressor Network is a Key Responder to Microenvironmental Components of Chronic Inflammatory Stress. Cancer Research 65, 10255-10264.
  • Hazel E. Warburton, et al. (2005) p53 regulation and function in renal cell carcinoma. Cancer Research 65, 6498-6503.
  • Urs Hobom and Matthias Dobbelstein. (2004) E1B-55-kilodalton protein is not required to block p53-induced transcription during adenovirus infection. Journal of Virology 78, 7685-7697.
    		•
    Application referencesImmunoprecipitation and Immunocytochemistry Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Paraffin Sections, Frozen Sections, Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Immunoblotting Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071.