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OP32 Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246)

This Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) is validated for use in Immunocytochemistry, Paraffin Sections, Immunoblotting for the detection of p53 (Ab-4) (Wild type).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP32
  
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Overview

Key Spec Table

Host
M
Description
OverviewRecognizes the ~53 kDa wild-type p53 protein in its native conformation in SV-T2 cells. Does not recognize mutant or denatured p53 protein.

This product has been discontinued.
Catalogue NumberOP32
Brand Family Calbiochem®
Application Data
Detection of mouse p53 by immunocytochemical staining. Sample: SV-T2 cells fixed in methanol. Primary antibody: Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) (Cat. No. OP32) (1 µg/ml). Detection: fluorescence with DAPI counterstain.
References
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994.Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controlSK-OV-3 cells
Positive controlSV-T2 cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesOriginal Clone Yewdell, J.W., et al. 1986. J. Virol. 59, 444.
Key Applications Immunocytochemistry
Immunoprecipitation
Not Western Blot
Paraffin Sections
Application NotesImmunocytochemistry (1-20 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
Immunoblotting (not recommended)
Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met (less than 1 h) will help reduce background. Anti-p53 (Ab-4) (Wild type), preferentially recognizes wild-type p53 of murine cellular origin; it will not react with mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenBALB/c SVA31 E7 cells
ImmunogenMouse
Epitopea conformational epitope within amino acids 85-109
ClonePAb246
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
OP32 0