Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PC409 Anti-p14ARF (Ab-1) (118-132) Rabbit pAb

This Anti-p14ARF (Ab-1) (118-132) Rabbit pAb is validated for use in Immunoblotting for the detection of p14ARF (Ab-1) (118-132).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC409
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
Overview

This product has been discontinued.




Recognizes the ~14 kDa p14ARF protein in Saos-2 cells. Sold under license of U.S. Patent 6,482,929.
Catalogue NumberPC409
Brand Family Calbiochem®
Application Data
Detection of human p14ARF by immunoblotting. Sample: Whole cell lysate from SAOS cells. Primary antibody: Anti-p14ARF (Ab-1) (118-132) Rabbit pAb (1:500). Detection: chemiluminescence.
References
ReferencesSharpless, N.E., and DePinho, R. A., 1999. Curr. Opin. Genet. Devel. 9, 22.
Bates, S., et al. 1998. Nature 395, 124.
Gorgoulis, V.G., et al. 1998. Am. J. Pathol. 153, 1749.
Pomerantz, J., et al. 1998. Cell 92, 713.
Stott, F.J., et al. 1998. EMBO J. 17, 5001.
Vonlanthen, S., et al. 1998. Oncogene 17, 2770.
Zhang, Y., et al. 1998. Cell 92, 725.
Product Information
DeclarationSold under license of U.S. Patent 6,482,929.
FormLiquid
FormulationUndiluted serum.
Positive controlSaos-2 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:500)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide [(K)AGRARCLGPSARGPG] corresponding to amino acids 118-132 of human p14ARF (p16β)
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC409 0

Documentation

Anti-p14ARF (Ab-1) (118-132) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-p14ARF (Ab-1) (118-132) Rabbit pAb Certificates of Analysis

TitleLot Number
PC409

References

Reference overview
Sharpless, N.E., and DePinho, R. A., 1999. Curr. Opin. Genet. Devel. 9, 22.
Bates, S., et al. 1998. Nature 395, 124.
Gorgoulis, V.G., et al. 1998. Am. J. Pathol. 153, 1749.
Pomerantz, J., et al. 1998. Cell 92, 713.
Stott, F.J., et al. 1998. EMBO J. 17, 5001.
Vonlanthen, S., et al. 1998. Oncogene 17, 2770.
Zhang, Y., et al. 1998. Cell 92, 725.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-October-2007 RFH
ApplicationImmunoblotting (1:500)
Application Data
Detection of human p14ARF by immunoblotting. Sample: Whole cell lysate from SAOS cells. Primary antibody: Anti-p14ARF (Ab-1) (118-132) Rabbit pAb (1:500). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~14 kDa p14ARF protein.
BackgroundThe human p14ARF protein (murine homologue is p19ARF) results from the translation of an alternative reading frame of the CDKN2A (p16INK4) gene. The α transcript gives rise to the p16 gene product while the β transcript gives rise to the p14ARF gene product. Although transcriptionally derived from shared exons, there is little amino acid sequence homology between the two proteins. The function of the p14ARF protein is to activate a p53 response and thereby induce cell cycle arrest. p53 in turn negatively regulates p14ARF expression. The mechanism of action for p14ARF is a direct binding of the COOH terminal domain of MDM2 which in turn forms a ternary complex with p53. Interaction with MDM2 results in the blocking of the MDM2 induced degradation of p53. p14ARF transcription is also modulated by the activity of E2F1 expression. Loss of p14ARF correlates with loss of p16 expression and alteration in expression of both proteins is frequently seen in non-small cell lung carcinoma.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide [(K)AGRARCLGPSARGPG] corresponding to amino acids 118-132 of human p14ARF (p16β)
IsotypeIgG
Specieshuman, not mouse, not rat
Positive controlSaos-2 cells
FormLiquid
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesSharpless, N.E., and DePinho, R. A., 1999. Curr. Opin. Genet. Devel. 9, 22.
Bates, S., et al. 1998. Nature 395, 124.
Gorgoulis, V.G., et al. 1998. Am. J. Pathol. 153, 1749.
Pomerantz, J., et al. 1998. Cell 92, 713.
Stott, F.J., et al. 1998. EMBO J. 17, 5001.
Vonlanthen, S., et al. 1998. Oncogene 17, 2770.
Zhang, Y., et al. 1998. Cell 92, 725.