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MAB11004 Anti-hnRNP Q Antibody, clone 7A11.2

Anti-hnRNP Q Antibody, clone 7A11.2 is a Mouse Monoclonal Antibody for detection of hnRNP Q also known as heterogeneous nuclear ribonucleoprotein Q-like & has been validated in WB, ICC.

Anti-hnRNP Q Antibody, clone 7A11.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: hnRNP-Q, hnRNP Q, SYNCRIP, HNRPQ, NSAP1 UniProt Number: O60506
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MAB11004
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB11004
DescriptionAnti-hnRNP Q Antibody, clone 7A11.2
Alternate Names
  • heterogeneous nuclear ribonucleoprotein Q-like
  • Synaptotagmin-binding, cytoplasmic RNA-interacting protein
  • Glycine- and tyrosine-rich RNA-binding protein
  • GRY-RBP
  • NS1-associated protein 1
Background InformationHeterogeneous nuclear ribonucleoprotein Q, commonly refered to as hnRNP Q, can be found as three different isoforms derived from alternative splicing of a single gene. This RNA binding protein is implicated in control of mRNA precursor splicing for the survival of motor neurons (SMN) protein. Loss-of-function mutations of SMN are linked to spinal muscular atrophy (SMA) a common neurodegenerative disease. It has been shown that hnRNP Q proteins interact with SMN, and this is interacton is required for efficient pre-mRNA splicing in vitro. Current data suggests that hnRNP Q is a splicing modulator of SMN.
References
Product Information
FormatPurified
Control
  • NIH/3T3 cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-hnRNP Q Antibody, clone 7A11.2 is a Mouse Monoclonal Antibody for detection of hnRNP Q also known as heterogeneous nuclear ribonucleoprotein Q-like & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected hnRNP Q on 10 µg of PC-12 and HEK293 cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected hnRNP Q in NIH/3T3, A431, and HeLa cells.
Biological Information
ImmunogenFull length recombinant protein corresponding to human hnRNPQ.
Clone7A11.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • hnRNP-Q
  • hnRNP Q
  • SYNCRIP
  • HNRPQ
  • NSAP1
Purification MethodProtein G
UniProt Number
UniProt SummaryFUNCTION: Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.

SUBUNIT STRUCTURE: Isoform 1 is a component of the APOB mRNA editosome complex and interacts with APOBEC1 and A1CF (APOBEC1 complementation factor). Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR, HNRPD and SYNCRIP. Isoform 3 interacts with HNRPR. Interacts with POLR2A hyperphosphorylated C-terminal domain. Interacts with minute virus of mice (MVM) NS1 protein. Isoform 1, isoform 2 and isoform 3 interact with SMN. Isoform 3 interacts through its C-terminal domain with SYT7, SYT8 and SYT9 (By similarity). The non-phosphorylated and phosphorylated forms are colocalized with PAIP1 in polysomes (By similarity). Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1 (By similarity). Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.

SUBCELLULAR LOCATION: Cytoplasm. Microsome (By similarity). Endoplasmic reticulum (By similarity). Nucleus (By similarity). Note: The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Isoform 1: Nucleus › nucleoplasm (By similarity). Note: Expressed predominantly in the nucleoplasm (By similarity).

Isoform 2: Nucleus › nucleoplasm (By similarity). Note: Expressed predominantly in the nucleoplasm (By similarity).

Isoform 3: Nucleus › nucleoplasm (By similarity). Note: Expressed predominantly in the nucleoplasm (By similarity).

TISSUE SPECIFICITY: Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.

Domain The domain containing eight Arg-Gly-Gly repeats may be involved in RNA-binding and protein-protein interactions.

PTM: Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR.

SEQUENCE SIMILARITIES: Contains 3 RRM (RNA recognition motif) domains.

SEQUENCE CAUTION: The sequence AAH15575.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence starting in position 413.
Molecular Weight~59 observed. Mulitple isoforms may be observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected hnRNP Q on 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB11004 04053252750229

Documentation

Anti-hnRNP Q Antibody, clone 7A11.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-hnRNP Q Antibody, clone 7A11.2 Certificates of Analysis

TitleLot Number
Anti-hnRNP Q, clone 7A11.2 - 2453291 2453291
Anti-hnRNP Q, clone 7A11.2 - 3146935 3146935
Anti-hnRNP Q, clone 7A11.2 - 3269455 3269455
Anti-hnRNP Q, clone 7A11.2 - 3385833 3385833
Anti-hnRNP Q, clone 7A11.2 - 3423235 3423235
Anti-hnRNP Q, clone 7A11.2 - 3746894 3746894
Anti-hnRNP Q, clone 7A11.2 - 3860929 3860929
Anti-hnRNP Q, clone 7A11.2 - 3975861 3975861
Anti-hnRNP Q, clone 7A11.2 - NRG1856990 NRG1856990
Anti-hnRNP Q, clone 7A11.2 -2586539 2586539
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