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07-421-I Anti-dimethyl Histone H3 (Lys27)

Anti-dimethyl Histone H3 (Lys27) Antibody, Cat. No. 07-421-I, is a highly specific chicken polyclonal antibody that targets histone H3 Lys27 dimethylation (H3K27me2) and has been tested in Dot Blot, Chromatin Immunoprecipitation, and Western Blotting.

Anti-dimethyl Histone H3 (Lys27) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FB, HIST1H3C, H3FC, HIST1H3D, H3FD, HIST1H3E, HIST1H3F, H3FF, HIST1H3G, HIST1H3H, H3FH, HIST1H3I, H3FI, HIST1H3J, H3FJ, H3FK, HIST3H3, H3FT, H3T UniProt Number: P84243
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07-421-I
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Ch, M, RWB, ChIP, DBChAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-421-I
DescriptionAnti-dimethyl Histone H3 (Lys27)
Alternate Names
  • H3K27me2
  • H3.3B
  • Lys27 dimethylated
  • H3 histone
  • family 3A
  • Lys27 dimethylated
  • H3 histone
  • family 3B
  • Lys27 dimethylated
  • Histone H3
  • Lys27 dimethylated
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
FormatAffinity Purified
PresentationPurified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-dimethyl Histone H3 (Lys27) Antibody, Cat. No. 07-421-I, is a highly specific chicken polyclonal antibody that targets histone H3 Lys27 dimethylation (H3K27me2) and has been tested in Dot Blot, Chromatin Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
  • Dot Blot
Application NotesChromatin Immunoprecipitation Analysis: 12 µL from a representative lot detected Lys27-dimethylated histone H3 occupancy at the -globin promoter region using HeLa nuclear extract (0.1 million cell eq. per ChIP) and Magna ChIP HiSens Chromatin Immunoprecipitation Kit (Cat. No. 17-10460).

Dot Blot Analysis: A 1:500 dilution from a representative lot specifically detected histone H3 peptide with K27me2 modification, but not H3 peptides with unmodified K27 and other K27 modifications, H2A, H2B, or H4 peptides by dot blot analysis using AbSurance H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665) and AbSurance H3 Antibody Specificity Array (Cat. No. 16-667).
Biological Information
ImmunogenKLH-conjugated peptide corresponding to amino acids 22-32 of human histone H3 with dimethylated Lys27.
ConcentrationPlease refer to lot specific datasheet.
HostChicken
SpecificitySpecificity of this chicken polyclonal antibody toward H3K27me2 modification has been confirmed using AbSurance H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665) and AbSurance H3 Antibody Specificity Array (Cat. No. 16-667). No cross-reactivity toward histone H3 peptides with unmodified K27 and other K27 modifications, H2A, H2B, or H4 peptides.
IsotypeIgY
Species Reactivity
  • Human
  • Chicken
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat, Chicken. Not Yeast. Broad species cross-reactivity expected based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FB
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FD
  • HIST1H3E
  • HIST1H3F
  • H3FF
  • HIST1H3G
  • HIST1H3H
  • H3FH
  • HIST1H3I
  • H3FI
  • HIST1H3J
  • H3FJ
  • H3FK
  • HIST3H3
  • H3FT
  • H3T
Modifications
  • Methylation
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~17 kDa observed. 15.20 kDa calculated (based on UniProt P84243 with Met1 removed). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Lys27-dimethylated histone H3 in 10 µg of HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-421-I 04054839034688