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OP31 Anti-c-Myc (Ab-3) Mouse mAb (33)

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OP31
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Av, H, MMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~60-67 kDa c-Myc protein HL60 cells.

Catalogue NumberOP31
Brand Family Calbiochem®
References
ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
Erisman, M.D., et al. 1985. Mol. Cell. Biol. 5, 1969.
Nau, M.N., et al. 1985. Nature 318, 69.
Persson, H., et al. 1984. Science 225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Dalla-Favera, R., et al. 1983. Science 219, 963.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlHT1080 cells
Positive controlHL-60 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Wafa, O., et al. 2002. Mol. Cell 9, 1031. Paraffin Sections Reichelt, J. and Magin, T.M. 2002. J. Cell Sci. 115, 2639.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Not Paraffin Sections
Application NotesFrozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (see comments and application references)
Application CommentsThe c-myc protein is extremely labile. c-myc can be degraded during a freeze/thaw cycle; thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. Recognizes full-length c-myc as well as c-myc cleavage products. The sequence of c-myc predicts a ~45 kDa protein, but c-myc migrates under reducing conditions as a ~64-67 kDa band. For staining paraffin sections, heat pretreatment in 10 mM citrate buffer, pH 6.0 is required (see application references). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human c-Myc
Clone33
HostMouse
IsotypeIgG₁
Species Reactivity
  • Avian
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP31 0

Documentation

Anti-c-Myc (Ab-3) Mouse mAb (33) SDS

Title

Safety Data Sheet (SDS) 

Anti-c-Myc (Ab-3) Mouse mAb (33) Certificates of Analysis

TitleLot Number
OP31

References

Reference overview
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
Erisman, M.D., et al. 1985. Mol. Cell. Biol. 5, 1969.
Nau, M.N., et al. 1985. Nature 318, 69.
Persson, H., et al. 1984. Science 225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Dalla-Favera, R., et al. 1983. Science 219, 963.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-August-2008 JSW
ApplicationFrozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (see comments and application references)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2 mouse myeloma cells. Recognizes the ~60-67 kDa (apparent MW) c-Myc protein.
BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. In both mouse plasmacytomas and human Burkitt's lymphoma, coding sequences from the c-myc gene are translocated from their normal chromosomal position into a transcriptionally active immunoglobulin locus on another chromosome. Amplification of the c-myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-myc gene has been found amplified in neuroblastomas, small cell lung carcinomas and Wilms tumors. L-myc is amplified in small cell lung cancer. Furthermore, elevated levels of myc mRNA and protein have been demonstrated in human tumors even in the absence of gene amplification. Immunological studies have shown that the human c-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
HostMouse
Immunogenrecombinant, human c-Myc
Clone33
IsotypeIgG₁
Speciesavian, human, mouse
Positive controlHL-60 cells
Negative controlHT1080 cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThe c-myc protein is extremely labile. c-myc can be degraded during a freeze/thaw cycle; thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. Recognizes full-length c-myc as well as c-myc cleavage products. The sequence of c-myc predicts a ~45 kDa protein, but c-myc migrates under reducing conditions as a ~64-67 kDa band. For staining paraffin sections, heat pretreatment in 10 mM citrate buffer, pH 6.0 is required (see application references). Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
Erisman, M.D., et al. 1985. Mol. Cell. Biol. 5, 1969.
Nau, M.N., et al. 1985. Nature 318, 69.
Persson, H., et al. 1984. Science 225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Dalla-Favera, R., et al. 1983. Science 219, 963.
Application referencesImmunoblotting Wafa, O., et al. 2002. Mol. Cell 9, 1031. Paraffin Sections Reichelt, J. and Magin, T.M. 2002. J. Cell Sci. 115, 2639.