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ABN2247-25UG Anti-altFUS

Anti-altFUS, Cat. No. ABN2247, is a rabbit polyclonal antibody that detects altFUS and is tested for use in Immunocytochemistry and Western Blotting.

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ABN2247-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberABN2247-25UG
DescriptionAnti-altFUS
Alternate Names
  • Alt-Fus
Background InformationAltFus (UniProt: A0A823ADP9; also known as Alt-Fus) is encoded by the FUS gene (Gene ID: 2521) in human. FUS is a nuclear RNA-binding protein with a C-terminal nuclear localization signal. It is involved in RNA processing, DNA repair, and cell proliferation. FUS is a dual coding gene that also codes for altFUS, a highly conserved, 170 amino acid protein that is endogenously expressed in many cell lines and tissues, including the motor cortex and motor neurons. Higher expression of FUS as well as altFUS contribute to neuronal toxicity. AltFUS is embedded within the FUS gene coding sequence. It is mainly a mitochondrial protein, but a weak cytoplasmic signal is also detected. Cells overexpressing altFUS display altered mitochondrial network, with a higher number of fragmented mitochondria. It can also lead to a loss in mitochondrial potential and accumulation of cytoplasmic aggregates. AltFUS is reported to inhibit autophagy and drive the accumulation of FUS- and TDP43-positive cytoplasmic aggregates. Suppression of its expression in a Drosophila model of FUS-related toxicity is shown to protect against neurodegeneration. (Ref.: Brunet, MA., et al. (3021). EMBO Rep. 22; e50640).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in PBS with 0.01% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-altFUS, Cat. No. ABN2247, is a rabbit polyclonal antibody that detects altFUS and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A representative lot detected altFUS in Immunocytochemistry applications (Brunet, M.A., et. al. (2021). EMBO Rep. 22(1); e50640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenLinear peptides corresponding to 19 amino acids from the N-terminal region and 15 amino acids from the internal region of mitochondrial specific human altFUS.
EpitopeInternal
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects a mitochondrial specific 170 amino acid proteins
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FUS
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~20 kDa observed; 17.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human brain tissue lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected altFUS in Human brain tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN2247-25UG 04065268978924