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GR37 Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4)

This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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GR37
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Am, Ch, Ht, H, M, Porcine, RRMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
GR37-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes both occupied and unoccupied vitamin D receptor in breast carcinomas expressing the vitamin D receptor protein.
Catalogue NumberGR37
Brand Family Calbiochem®
References
ReferencesBrown, A.J. 1999. Nephrol. Dial. Transplant. 14, 11.
Fleet, J.C. 1999. Nutr. Rev. 57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res. 4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem. 46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res. 47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 241.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlBreast carcinoma tissue (see comments)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Gel Shift
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (1:50-1:100)
Gel Shift (see comments)
Immunoblotting (1:200-1:400)
Immunoprecipitation (10 µl/mg protein lysate)
Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
Application CommentsDoes not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified, chick intestine vitamin D receptor
ImmunogenChicken
Clone9A7γE10.4
HostRat
IsotypeIgG₁
Species Reactivity
  • Amphibian
  • Chicken
  • Hamster
  • Human
  • Mouse
  • Porcine
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GR37-100UG 04055977228311

Documentation

Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) SDS

Title

Safety Data Sheet (SDS) 

Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) Certificates of Analysis

TitleLot Number
GR37

References

Reference overview
Brown, A.J. 1999. Nephrol. Dial. Transplant. 14, 11.
Fleet, J.C. 1999. Nutr. Rev. 57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res. 4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem. 46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res. 47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 241.

Citations

Title
  • Roberto Paredes, et al. (2004) Bone-Specific Transcription Factor Runx2 Interacts with the 1α,25-Dihydroxyvitamin D3 Receptor To Up-Regulate Rat Osteocalcin Gene Expression in Osteoblastic Cells. Molecular and Cellular Biology 24, 8847-8861.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision27-August-2007 RFH
    ApplicationFrozen Sections (1:50-1:100)
    Gel Shift (see comments)
    Immunoblotting (1:200-1:400)
    Immunoprecipitation (10 µl/mg protein lysate)
    Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)
    DescriptionPurified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with mouse myeloma p3 NS 1/Ag4-1 (NS1) mouse myeloma cells. Recognizes both the occupied and unoccupied forms of vitamin D receptor.
    BackgroundVitamin D Receptor (VDR) also known as 1,25-dihydroxyvitamin D3 receptor is a member of the nuclear steroid hormone receptor superfamily which regulate gene transcription by interacting with response elements in gene promoters. VDR binds with the retinoid X receptor (RXR) from specific DNA sequences in the promoter region of vitamin D response elements (VDREs). VDR has been implicated in a variety of physiological functions including effects on growth and differentiation in normal and malignant cells. These effects are mediated through a single ligand, calcitriol. Cacitriol the active metabolite of vitamin D is a known regulator of calcium homeostasis but it also has numerous other functions including inhibition of proliferation of cancer cells and suppression of T-cell proliferation and cytokine production. Human breast carcinomas, leukemias, and prostatic carcinoma cells all express vitamin D receptor, suggesting that vitamin D may have beneficial actions on various malignancies that may prove useful in chemoprevention and/or differentiation therapy.
    HostRat
    Immunogen speciesChicken
    Immunogenpurified, chick intestine vitamin D receptor
    Clone9A7γE10.4
    IsotypeIgG₁
    Speciesamphibian, chicken, hamster, human, mouse, porcine, rat
    Positive controlBreast carcinoma tissue (see comments)
    FormLiquid
    FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsDoes not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial use, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesBrown, A.J. 1999. Nephrol. Dial. Transplant. 14, 11.
    Fleet, J.C. 1999. Nutr. Rev. 57 60.
    Evans, S.R., et al. 1998. Clin. Cancer Res. 4, 1591.
    Friedrich, M., et al. 1998. J. Histochem. Cytochem. 46, 1335.
    Issa, L.L., et al. 1998. Inflamm. Res. 47, 451.
    Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 121.
    Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol. 66, 241.
    Citation
  • Roberto Paredes, et al. (2004) Bone-Specific Transcription Factor Runx2 Interacts with the 1α,25-Dihydroxyvitamin D3 Receptor To Up-Regulate Rat Osteocalcin Gene Expression in Osteoblastic Cells. Molecular and Cellular Biology 24, 8847-8861.
    		•