Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

IF01 Anti-Vimentin/LN6 (Ab-1) Mouse mAb (V-9)

Anti-Vimentin/LN6 (Ab-1) Mouse mAb (V-9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
IF01
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ch, H, Porcine, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~58 kDa vimentin protein. Does not cross-react with closely related intermediate filament proteins including desmin and GFA.
Catalogue NumberIF01
Brand Family Calbiochem®
References
ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
Osborn, M., et al. 1984. In Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
Geisler, N., et al. 1983. FEBS 163, 22.
Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
Osborn, M. and Weber, K. 1982. Cell 31, 303.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controlCEM-CCRF cells
Positive controlHeLa, IMR90, or WI38 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesCross-Reactivity Osborn, M., et al. 1984. Eur. J. Cell Biol. 34, 137. Immunostaining Caselitz, J., et al. 1983. Virch. Arch. 400, 43.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Paraffin Sections
Application NotesFrozen sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml, indirect)
Paraffin sections (1-5 µg/ml)
Application CommentsDoes not cross-react with closely related intermediate filament proteins including desmin and GFA (see application references). This antibody stains intermediate filaments found in chondrocytes, fibroblasts, and connective tissue cells, as well as those found in fibroblast-derived cell lines (such as human WI38) and in certain classes of human tumors, including malignant melanomas (see application references). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified porcine vimentin
ImmunogenPorcine
CloneV-9
HostMouse
IsotypeIgG₁
Species Reactivity
  • Chicken
  • Human
  • Porcine
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IF01 0

Documentation

Anti-Vimentin/LN6 (Ab-1) Mouse mAb (V-9) Certificates of Analysis

TitleLot Number
IF01

References

Reference overview
Droese, M., et al. 1984. Acta. Cytol. 28, 368.
Osborn, M., et al. 1984. In Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
Geisler, N., et al. 1983. FEBS 163, 22.
Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
Osborn, M. and Weber, K. 1982. Cell 31, 303.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-May-2009 JSW
ApplicationFrozen sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml, indirect)
Paraffin sections (1-5 µg/ml)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with PAI mouse myeloma cells. Recognizes the ~58 kDa vimentin protein.
BackgroundIntermediate (10 nm) filaments are present in, and provide information concerning the origin of most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (GFA), skeletal, visceral and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). A few well-defined cell types coexpress vimentin along with one other intermediate filament type. Vimentin (Ab-1) stains the vimentin subclass of intermediate filaments in human tissues.
HostMouse
Immunogen speciesPorcine
Immunogenpurified porcine vimentin
CloneV-9
IsotypeIgG₁
Specieschicken, human, not mouse, porcine, rat
Positive controlHeLa, IMR90, or WI38 cells
Negative controlCEM-CCRF cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with closely related intermediate filament proteins including desmin and GFA (see application references). This antibody stains intermediate filaments found in chondrocytes, fibroblasts, and connective tissue cells, as well as those found in fibroblast-derived cell lines (such as human WI38) and in certain classes of human tumors, including malignant melanomas (see application references). Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
Osborn, M., et al. 1984. In Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
Geisler, N., et al. 1983. FEBS 163, 22.
Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
Osborn, M. and Weber, K. 1982. Cell 31, 303.
Application referencesCross-Reactivity Osborn, M., et al. 1984. Eur. J. Cell Biol. 34, 137. Immunostaining Caselitz, J., et al. 1983. Virch. Arch. 400, 43.