Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABS1679-25UL Anti-VPS28

Anti-VPS28, Cat. No. ABS1679, is a rabbit polyclonal antibody that detects ESCRT-I complex subunit VPS28 and has been tested for use in Immunofluorescence and Western Blotting.

Anti-VPS28 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: VPS28 UniProt Number: Q9UK41
View Products on Sigmaaldrich.com
ABS1679-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABS1679-25UL
DescriptionAnti-VPS28
Alternate Names
  • Vacuolar protein sorting-associated protein 28 homolog
  • H-Vps28
  • ESCRT-I complex subunit VPS28
Background InformationVacuolar protein sorting-associated protein 28 homolog (UniProt: Q9UK41; also known as H-Vps28, ESCRT-I complex subunit VPS28) is encoded by the VPS28 gene (Gene ID: 51160) in human. VPS28 is a component of the Endosomal Sorting Complex Required for Transport (ESCRT)-I complex that regulates the vesicular trafficking process. This complex forms the machinery driving protein sorting from endosomes to lysosomes. It is located in the cell membrane and late endosome membrane. Two isoforms of VPS28 have been described that are generated by alternative splicing. ESCRT complexes play a vital role in receptor down-regulation, lysosome biogenesis, and budding of HIV. In humans, ESCRT-I complex consists of TSG101, VPS28, and one of four potential human VPS37 homologues. The main role of ESCRT-I is to recognize ubiquitinated cargo via the UEV domain of the VPS23/TSG101 subunit. The N-terminal domain of VPS28 contains a conformationally dynamic hydrophobic binding site on its surface. The C-terminal domain of VPS28 is reported to bind the ESCRT-II complex. (Ref.: Kostelansky, MS et al. (2006). Cell 125(1); 113-126).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-VPS28, Cat. No. ABS1679, is a rabbit polyclonal antibody that detects ESCRT-I complex subunit VPS28 and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected recombinant VPS28  protein and VPS28 in  cell extracts (Stuchell, M.D., et. al. (2004). J Biol Chem. 279(34):36059-71). Immunofluorescence Analysis: A representative lot detected VPS28 in HEK293T cells transfected with VPS28 (Silvestri, L.S., et. al. (2007). J Infect Dis. 196 Suppl 2:S264-70).
Biological Information
ImmunogenA full-length recombinant ESCRT-I complex subunit VPS28 (VPS28).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects ESCRT-I complex subunit VPS28 in human cells.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Gene Symbol
  • VPS28
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~51 kDa observed; 25.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in GST-VPS28 recombinant protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant GST-VPS28 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1679-25UL 04054839499203