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NA14 Anti-Topoisomerase II (Ab-1) Mouse mAb (SWT3D1)

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NA14
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes ~170 kDa topoisomerase II protein in HeLa cells and breast carcinoma tissue.
Catalogue NumberNA14
Brand Family Calbiochem®
References
Referencesvan der Zee, A.G., et al. 1994. Ann. Oncol. 5, 75.
Cummings, J. and Smyth, J.F. 1993. Ann. Oncol. 4, 533.
Robinson, R.G., et al. 1993. Hybridoma 12, 407.
Anderson, H.J. and Roberge, M. 1992. Cell Biol. Intl. Reports 16, 717.
Gasser, S.M., et al. 1992. J. Antonie Van Leeuwenhoek 62, 15.
Tan, K.B., et al. 1992. Can. Res. 52, 231.
Osheroff, N., et al. 1991. Bioessays 13, 269.
Chung, T.D.Y., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 9431.
Wang, J.C. 1985. Ann. Rev. Biochem. 54, 665.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 2% gelatin.
Negative controlP388D cells
Positive controlHeLa cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Robinson, R.G., et al. 1993. Hybridoma 12, 407.
Key Applications Immunoblotting (Western Blotting)
Not Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (5 µg/ml, see application references)
Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
Immunoprecipitation (not recommended)
Application CommentsHas a high affinity for topoisomerase IIα and a low to moderate affinity to topoisomerase IIβ. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human topoisomerase IIα
ImmunogenHuman
CloneSWT3D1
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NA14 0

Documentation

Anti-Topoisomerase II (Ab-1) Mouse mAb (SWT3D1) SDS

Title

Safety Data Sheet (SDS) 

Anti-Topoisomerase II (Ab-1) Mouse mAb (SWT3D1) Certificates of Analysis

TitleLot Number
NA14

References

Reference overview
van der Zee, A.G., et al. 1994. Ann. Oncol. 5, 75.
Cummings, J. and Smyth, J.F. 1993. Ann. Oncol. 4, 533.
Robinson, R.G., et al. 1993. Hybridoma 12, 407.
Anderson, H.J. and Roberge, M. 1992. Cell Biol. Intl. Reports 16, 717.
Gasser, S.M., et al. 1992. J. Antonie Van Leeuwenhoek 62, 15.
Tan, K.B., et al. 1992. Can. Res. 52, 231.
Osheroff, N., et al. 1991. Bioessays 13, 269.
Chung, T.D.Y., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 9431.
Wang, J.C. 1985. Ann. Rev. Biochem. 54, 665.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
ApplicationImmunoblotting (5 µg/ml, see application references)
Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
Immunoprecipitation (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63Ag8.653 mouse myeloma cells. Recognizes the ~170 kDa topoisomerase II protein.
BackgroundTopoisomerase II is a nuclear enzyme whose primary function is to induce temporary double-strand breaks in DNA and to facilitate the passage of the duplex DNA segments through these breaks. Phosphorylation of topoisomerase II in a cell cycle regulated manner appears to be important for progression through the cell cycle. The enzyme exists as two discrete isozymes, topoisomerase IIα (~170 kDa) and topoisomerase IIβ (~180 kDa) which are under independent transcriptional regulation. Both isozymes have been identified in human and mouse cells but only the topoisomerase IIα form appears in yeast. The gene encoding topoisomerase IIα has been mapped to chromosome 17 and is in close proximity to the gene for erbB-2. Altered expression of topoisomerase II has been documented in many cancers including AML, CLL, small cell lung cancer, breast and ovarian cancer, and colon cancer. Based on this observation, topoisomerase II has been identified as the target of many widely used antineoplastic drugs as well as the target of rational drug design.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant human topoisomerase IIα
CloneSWT3D1
IsotypeIgG₁
Specieshuman, not mouse, not rat
Positive controlHeLa cells or breast carcinoma tissue
Negative controlP388D cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsHas a high affinity for topoisomerase IIα and a low to moderate affinity to topoisomerase IIβ. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
Referencesvan der Zee, A.G., et al. 1994. Ann. Oncol. 5, 75.
Cummings, J. and Smyth, J.F. 1993. Ann. Oncol. 4, 533.
Robinson, R.G., et al. 1993. Hybridoma 12, 407.
Anderson, H.J. and Roberge, M. 1992. Cell Biol. Intl. Reports 16, 717.
Gasser, S.M., et al. 1992. J. Antonie Van Leeuwenhoek 62, 15.
Tan, K.B., et al. 1992. Can. Res. 52, 231.
Osheroff, N., et al. 1991. Bioessays 13, 269.
Chung, T.D.Y., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 9431.
Wang, J.C. 1985. Ann. Rev. Biochem. 54, 665.
Application referencesImmunoblotting Robinson, R.G., et al. 1993. Hybridoma 12, 407.