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OP185 Anti-Toll-Like Receptor 9 (Ab-1) Mouse mAb (26C593)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TLR9

OP185
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~120 kDa TLR9 protein in human peripheral blood mononuclear cells and Ramos.
Catalogue NumberOP185
Brand Family Calbiochem®
SynonymsAnti-TLR9
Application Data
Detection of toll-like receptor 9 by immunoblotting. Samples: Tissue lysates from human PBMC (lane 1), human intestine (lane 2), mouse intestine (lane 3), and rat intestine (lane 4). Primary antibody: Anti-Toll-Like Receptor 9 (Ab-1) Mouse mAb (26C593) (Cat. No. OP185) (3 µg/ml). Detection: chemiluminescence.
References
ReferencesChuang, T.H., and Ulevitch, R.J., 2001. Biochim. Biophys. Acta 1518, 157.
Hemmi, H., et al. 2000. Nature 408, 740.
Takeuchi, O., et al. 1999 Gene 231, 59.
Muzio, M., et al. 1998. J. Exp. Med. 187, 2097.
Medzhitov, R., and Janeway, C.A., 1997. Cell 91, 295.
Product Information
FormLiquid
FormulationIn PBS containing 0.2% gelatin.
Positive controlHuman peripheral blood mononuclear cell lysate or Ramos cells
Preservative≤0.1% sodium azide
Applications
Key Applications Flow Cytometry
Immunoblotting (Western Blotting)
Application NotesFlow Cytometry (assay dependent)
Immunoblotting (assay dependent)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids in a unique region of toll-like receptor 9, conjugated to KLH
ImmunogenHuman
Clone26C593
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP185 0

Documentation

Anti-Toll-Like Receptor 9 (Ab-1) Mouse mAb (26C593) SDS

Title

Safety Data Sheet (SDS) 

Anti-Toll-Like Receptor 9 (Ab-1) Mouse mAb (26C593) Certificates of Analysis

TitleLot Number
OP185

References

Reference overview
Chuang, T.H., and Ulevitch, R.J., 2001. Biochim. Biophys. Acta 1518, 157.
Hemmi, H., et al. 2000. Nature 408, 740.
Takeuchi, O., et al. 1999 Gene 231, 59.
Muzio, M., et al. 1998. J. Exp. Med. 187, 2097.
Medzhitov, R., and Janeway, C.A., 1997. Cell 91, 295.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
SynonymsAnti-TLR9
ApplicationFlow Cytometry (assay dependent)
Immunoblotting (assay dependent)
Application Data
Detection of toll-like receptor 9 by immunoblotting. Samples: Tissue lysates from human PBMC (lane 1), human intestine (lane 2), mouse intestine (lane 3), and rat intestine (lane 4). Primary antibody: Anti-Toll-Like Receptor 9 (Ab-1) Mouse mAb (26C593) (Cat. No. OP185) (3 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~120 kDa TLR9 protein.
BackgroundThe mammalian Toll-like receptor (TLR) family is comprised of a group of transmembrane proteins characterized by multiple copies of leucine-rich repeats in the extracellular domain, and an IL-1 receptor motif in the cytoplasmic domain. TLRs signal through adaptor molecules and may be an important component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity and is essential to microbial recognition. TLRs characterized in the past have been shown to activate the MyD88/interleukin -1 receptor-associated kinase (IRAK) signaling pathway. Ten homologs of TLRs (TLR1-TLR10) have previously been described and the cDNA coding for TLR9 has been identified and isolated. Research has shown that TRL9 acts as a receptor for unmethylated CpG dinucleotides in bacterial DNA. Human and mouse TLR9 share an amino acid identity of 75.5%, and it is highly expressed in the spleen.
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids in a unique region of toll-like receptor 9, conjugated to KLH
Clone26C593
IsotypeIgG₁
Specieshuman
Positive controlHuman peripheral blood mononuclear cell lysate or Ramos cells
FormLiquid
FormulationIn PBS containing 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesChuang, T.H., and Ulevitch, R.J., 2001. Biochim. Biophys. Acta 1518, 157.
Hemmi, H., et al. 2000. Nature 408, 740.
Takeuchi, O., et al. 1999 Gene 231, 59.
Muzio, M., et al. 1998. J. Exp. Med. 187, 2097.
Medzhitov, R., and Janeway, C.A., 1997. Cell 91, 295.