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MABC1765-100UL Anti-TfR1 (CD71) Antibody, clone 3F3-FMA

Anti-TfR1 (CD71), clone 3F3-FMA, Cat. No. MABC1765, is a mouse monoclonal antibody that detects Tfr1 and is an excellent ferroptosis marker. It is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

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MABC1765-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABC1765-100UL
DescriptionAnti-TfR1 (CD71) Antibody, clone 3F3-FMA
Alternate Names
  • TR
  • TfR
  • TfR1
  • Trfr
  • T9
  • p90
  • CD71
Background InformationTransferrin receptor protein 1 (UniProt: Q62351; also known as TR, TfR, TfR1, Trfr, T9, p90, CD71) is encoded by the Tfrc (also known as Trfr) gene (Gene ID: 7037) in human. TfR1 is a single-pass type II membrane glycoprotein with a cytoplasmic domain (aa 1-67), a transmembrane domain (aa 68-88), and a long extracellular domain (aa 89-760). It can also be proteolytically cleaved at Arg100 to generate the soluble serum form (sTfR). TfR1 is involved in the uptake of iron via receptor mediated endocytosis into specialized endosomes where acidification leads to release of iron. TfR1 is reported to be essential for erythrocyte development and positive regulation of T and B cell proliferation through iron uptake. It also acts as a lipid sensor that regulates mitochondrial fusion by regulating the activation of the JNK pathway and degradation of mitofusin MFN2. Under conditions of low dietary levels of stearate (C18:0), it promotes the activation of JNK pathway that results in HUWE1-mediated ubiquitination and degradation of mitofusin MFN2 and inhibition of mitochondrial fusion. Clone 3F3-FMA is reported to be an effective ferroptosis-staining agent. It stains plasma membrane and the perinuclear region associated with the Golgi and the endosomal recycling compartment. (Ref.: Senilymaz, D., et al. (2015). Nature. 525(7567); 124-128; Feng, H., et al. (2020). Cell Rep. 30(10); 3411-3423).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-TfR1 (CD71), clone 3F3-FMA, Cat. No. MABC1765, is a mouse monoclonal antibody that detects Tfr1 and is an excellent ferroptosis marker. It is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesFlow Cytometry Analysis: A representative lot detected TfR1 (CD71) in Flow Cytometry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TfR1 (CD71) in HT-1080 cells.

Immunohistochemistry Applications: A representative lot detected TfR1 (CD71) in Immunohistochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

Immunocytochemistry Analysis: A representative lot detected TfR1 (CD71) in Immunocytochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

Western Blotting Analysis: A representative lot detected TfR1 (CD71) in Western Blotting applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified membrane fractions from OCI-LY7 cells.
EpitopeUnknown
Clone3F3-FMA
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 3F3-FMA is a mouse monoclonal antibody that detects human Transferrin receptor protein 1.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tfrc
  • Trfr
Purification MethodProtein G purified
UniProt Number
Molecular Weight~90 kDa observed; 84.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HT-1080 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TfR1 (CD71) in HT-1080 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1765-100UL 04065265944663

Documentation

Anti-TfR1 (CD71) Antibody, clone 3F3-FMA SDS

Title

Safety Data Sheet (SDS) 

Anti-TfR1 (CD71) Antibody, clone 3F3-FMA Certificates of Analysis

TitleLot Number
Anti-TfR1 (CD71), clone 3F3-FMA - 3815820 3815820
Anti-TfR1 (CD71), clone 3F3-FMA - 3821644 3821644
Anti-TfR1 (CD71), clone 3F3-FMA - 3822341 3822341
Anti-TfR1 (CD71), clone 3F3-FMA - 3884348 3884348
Anti-TfR1 (CD71), clone 3F3-FMA - 3923511 3923511
Anti-TfR1 (CD71), clone 3F3-FMA - 3939593 3939593
Anti-TfR1 (CD71), clone 3F3-FMA - 3944557 3944557
Anti-TfR1 (CD71), clone 3F3-FMA - 3959561 3959561
Anti-TfR1 (CD71), clone 3F3-FMA - 3983452 3983452
Anti-TfR1 (CD71), clone 3F3-FMA - 3995835 3995835
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