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ABN1721-25UG Anti-TMEM100

Anti-TMEM100, Cat. No. ABN1721, is a highly specific rabbit polyclonal antibody that targets Transmembrane protein 100 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Research Category: Neuroscience Gene Symbol: Tmem100 UniProt Number: Q9CQG9
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ABN1721-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIF, IH(P), IP, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1721-25UG
DescriptionAnti-TMEM100
Alternate Names
  • Transmembrane protein 100
Background InformationTransmembrane protein 100 (UniProt: Q9CQG9; also known as TMEM100) is encoded by the Tmem100 gene (Gene ID: 67888) in murine species. TMEM100 is a multi-pass membrane protein that is shown to be involved in renal development, vasculogenesis and lung cancer invasiveness. It plays a role during embryonic arterial endothelium differentiation and vascular morphogenesis through the ACVRL1 receptor-dependent signaling pathway upon stimulation by bone morphogenetic proteins, such as GDF2/BMP9 and BMP10. TMEM100 is also involved in the regulation of nociception by forming a complex with TRPA1 and TRPV1 and act as a modulator of their interaction in dorsal root ganglia (DRG) neurons. It is reported to weaken TRPA1 and TRPV1 interaction, thereby releasing the inhibition of TRPA1 by TRPV1 and increasing the single-channel open probability of the TRPA1-TRPV1 complex. TMEM100-deficient mice show a reduction in inflammatory mechanical hyperalgesia and TRPA1- but not TRPV1-mediated pain. A mutation involving aa 117-119 KRR to QQQ is shown to abolish TMEM100 interaction with TRPA1, but increases interaction with TRPV1. Mutations is Tmem100 gene can lead to embryonic lethality due to impaired differentiation of arterial endothelium and defects of vascular morphogenesis. (Ref.: Weng, H-J., et al. (2015). Neuron 85(4), 833-846).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-TMEM100, Cat. No. ABN1721, is a highly specific rabbit polyclonal antibody that targets Transmembrane protein 100 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected TMEM100 in mouse colon, rat peripheral nerve, and human cerebral cortex tissues.
Immunofluorescence Analysis: A representative lot detected TMEM100 in DRGs (Weng, H.J., et. al. (2015). Neuron. 85(4):833-46).
Western Blotting Analysis: A representative lot detected TMEM100 in DRG from cervical to lumbar levels (Weng, H.J., et. al. (2015). Neuron. 85(4):833-46).
Immunoprecipitation Analysis:  representative lot detected TMEM100 in whole-cell DRG or CHO cells lysates (Weng, H.J., et. al. (2015). Neuron. 85(4):833-46).
Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected TMEM100 in Dorsal root ganglion (DRG) of WT & TMEM100 CKO mice  (Courtesy of Drs. Hao-Jui Weng and Xinzhong Dong at Johns Hopkins University).
Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected TMEM100 in Dorsal root ganglion (DRG) sections from Avil-Cre;Tmem100+/+ (Wild Type mice) and Avil-Cre;Tmem100fl/fl (Tmem100 CKO) mice   (Courtesy of Drs. Hao-Jui Weng and Xinzhong Dong at Johns Hopkins University).
 
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminus region of murine Transmembrane protein 100 (TMEM100).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects transmembrane protein 100 (TMEM100) in murine cells. It targets an epitope with the 19 amino acids from the N-terminal end.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypePolyclonal Antibody
Gene Symbol
  • Tmem100
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~17 kDa observed; 14.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse lung tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TMEM100 in 10 µg of mouse lung tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1721-25UG 04054839490064

Documentation