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IM63 Anti-TIMP-1 (Ab-4) Mouse mAb (102D1)

Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tissue Inhibitor of Metalloproteinase-1

IM63
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~28.5 kDa TIMP-1 protein in endothelial cells, fibroblasts, and breast carcinoma tissue.
Catalogue NumberIM63
Brand Family Calbiochem®
SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
Application Data
Detection of human TIMP-1 by immunohistochemistry. Sample: Human breast carcinoma tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) (Cat. No. IM63) (1 µg/ml). Detection: fluorescence.

Detection of human TIMP-1 by immunoblotting. Sample: Human TIMP-1. Primary antibody: Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) (Cat. No. IM63) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesHerbst, H., et al. 1997. Am. J. Pathol. 150, 1647.
Jung, K., et al. 1997. Int. J. Cancer 74, 220.
Rivera, S., et al. 1997. J. Neurosci. 17, 4223.
Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
Hurskainen, T., et al. 1996. J. Histochem. Cytochem. 44, 1379.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
Product Information
DeclarationNot available for sale in Japan.
FormLiquid
FormulationIn 10 mM PBS, pH 7.4.
Positive controlCytotrophoblastic columns, endothelial or fibroblastic stromal cells of villi, large decidualized cells of decidual membrane in placenta, or breast carcinoma
PreservativeNone
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (1:100)
Immunoblotting (1:200-1:400, chemiluminescence)
Paraffin Sections (1:50, heat pre-treatment required)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human TIMP-1
ImmunogenHuman
Clone102D1
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM63 0

Documentation

Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) Certificates of Analysis

TitleLot Number
IM63

References

Reference overview
Herbst, H., et al. 1997. Am. J. Pathol. 150, 1647.
Jung, K., et al. 1997. Int. J. Cancer 74, 220.
Rivera, S., et al. 1997. J. Neurosci. 17, 4223.
Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
Hurskainen, T., et al. 1996. J. Histochem. Cytochem. 44, 1379.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-February-2009 JSW
SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
ApplicationFrozen Sections (1:100)
Immunoblotting (1:200-1:400, chemiluminescence)
Paraffin Sections (1:50, heat pre-treatment required)
Application Data
Detection of human TIMP-1 by immunohistochemistry. Sample: Human breast carcinoma tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) (Cat. No. IM63) (1 µg/ml). Detection: fluorescence.

Detection of human TIMP-1 by immunoblotting. Sample: Human TIMP-1. Primary antibody: Anti-TIMP-1 (Ab-4) Mouse mAb (102D1) (Cat. No. IM63) (1 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p3-X63-Ag8.653 mouse myeloma cells. Recognizes the ~28-29 kDa TIMP-1 protein.
BackgroundThe activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a ~28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9 and MMP-1 interacting through the catalytic domain of the MMP protein. The inhibition is via reversible non-covalent binding to form a 1:1 complex with the MMP and having a Kd of approximately 10-10
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human TIMP-1
Clone102D1
IsotypeIgG₁
Specieshuman
Positive controlCytotrophoblastic columns, endothelial or fibroblastic stromal cells of villi, large decidualized cells of decidual membrane in placenta, or breast carcinoma
FormLiquid
FormulationIn 10 mM PBS, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHerbst, H., et al. 1997. Am. J. Pathol. 150, 1647.
Jung, K., et al. 1997. Int. J. Cancer 74, 220.
Rivera, S., et al. 1997. J. Neurosci. 17, 4223.
Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
Hurskainen, T., et al. 1996. J. Histochem. Cytochem. 44, 1379.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 99.