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MABE1994-25UL Anti-TFAM Antibody, clone 20G2C12

Anti-TFAM, clone 20G2C12, Cat. No. MABE1994, is a mouse monoclonal antibody that detects Transcription factor A, mitochondrial (TFAM) and is tested for use in ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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MABE1994-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABE1994-25UL
DescriptionAnti-TFAM Antibody, clone 20G2C12
Alternate Names
  • Transcription factor A mitochondrial
  • mtTFA
  • Mitochondrial transcription factor 1
  • MtTF1
  • Transcription factor 6
  • TCF-6
  • Transcription factor 6-like 2
Background InformationTranscription factor A, mitochondrial (UniProt: Q00059; also known as mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM) is encoded by the TFAM (also known as TCF6, TCF6L2) gene (Gene ID: 7019) in human. TFAM is a DNA binding protein that plays multiple roles in regulating mitochondrial DNA function. As a sequence-specific transcription factor, it binds upstream of the light strand promoter and heavy strand promoter 1 to activate initiation of transcription. It is a component of the mitochondrial transcription initiation complex (composed of TFB2M, TFAM and POLRMT). In this complex, TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. TFAM contains two HMG boxes (aa 50-118 and 155-219). Phosphorylation by protein kinase A within the HMG box 1 is shown to impair its DNA binding and promotes its degradation by AAA+ Lon protease. Mutations in TFAM gene are known to cause hepatocerebral type of mitochondrial DNA depletion syndrome that is characterized by severe intrauterine growth restriction, neonatal-onset hypoglycemia and liver dysfunction. In this disease hepatic pathology includes cirrhosis, steatosis and cholestasis that leads to liver failure. (Ref.: Wang, YE., et al. (2013). PLoS ONE. 8(8); e74513).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-TFAM, clone 20G2C12, Cat. No. MABE1994, is a mouse monoclonal antibody that detects Transcription factor A, mitochondrial (TFAM) and is tested for use in ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • ChIP-seq
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesChIP-seq Analysis: A representative lot detected TFAM in ChIP-seq applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).

Immunoprecipitation Analysis: A representative lot immunoprecipitated TFAM in Immunoprecipitation applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TFAM in HeLa cells.

Western Blotting Analysis: A representative lot detected TFAM in Western Blotting applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).

Immunocytochemistry Analysis: A representative lot detected TFAM in Immunocytochemistry applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant human Transcription factor A, mitochondrial (TFAM).
EpitopeUnknown
Clone20G2C12
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 20G2C12 is a mouse monoclonal antibody that detects human mitochondrial Transcription Factor A (TFAM).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TFAM
  • TCF6
  • TCF6L2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~25 kDa observed; 29.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TFAM in HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1994-25UL 04065265872751