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MABS1997-25UG Anti-ST8SIA1 Antibody, clone HIC0-3C5

Anti-ST8SIA1, clone HIC0-3C5, Cat. No. MABS1997, is a mouse monoclonal antibody that detects Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase and has been tested for use in Flow Cytometry, Immunoflurescence, and Immunohistochemistry.

Anti-ST8SIA1 Antibody, clone HIC0-3C5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: ST8SIA1, SIAT8, SIAT8A UniProt Number: Q92185
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MABS1997-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IF, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1997-25UG
DescriptionAnti-ST8SIA1 Antibody, clone HIC0-3C5
Alternate Names
  • Alpha-N-acetylneuraminide alpha-2
  • 8-sialyltransferase
  • EC: 2.4.99.8
  • Alpha-2
  • 8-sialyltransferase 8A
  • Ganglioside GD3 synthase
  • Ganglioside GT3 synthase
  • Sialyltransferase 8A
  • SIAT8-A
  • Sialyltransferase St8Sia I
  • ST8SiaI
Background InformationAlpha-N-acetylneuraminide alpha-2,8-sialyltransferase (UniProt: Q92185; also known as EC: 2.4.99.8, Alpha-2,8-sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, Sialyltransferase 8A, SIAT8-A, Sialyltransferase St8Sia I, ST8SiaI) I encoded by the ST8SIA1 (also known as SIAT8, SIAT8A) gene (Gene ID: 6489) in human. SIAT8-A is a single-pass type II membrane protein that is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung. It is involved in the production of gangliosides GD3 and GT3 from GM3. It catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3 in a successive manner. It is also involved in the pathway protein glycosylation. SIAT8-A contains a cytoplasmic domain (aa 1-29); a transmembrane domain (aa 30-48); and a lumenal domain (aa 49-356). Two isoforms of SIAT8-A have been described that are produced by alternative splicing. (Ref.: Dorrell, C., et al. (2016). Nat. Commun. 7; 11756).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Applications
ApplicationAnti-ST8SIA1, clone HIC0-3C5, Cat. No. MABS1997, is a mouse monoclonal antibody that detects Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase and has been tested for use in Flow Cytometry, Immunoflurescence, and Immunohistochemistry.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry
Application NotesFlow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).

Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Biological Information
ImmunogenTrypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.
CloneHIC0-3C5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).
IsotypeIgMκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ST8SIA1
  • SIAT8
  • SIAT8A
UniProt Number
Molecular Weight40.52 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human pancreatic tissue sections.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected ST8SIA1 in human pancreatic tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1997-25UG 04054839540165