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ABN2264-10UL Anti-SNTF (cleaved spectrin)

Anti-SNTF (cleaved spectrin), Cat. No. ABN2264, is a highly specific rabbit polyclonal antibody that targets cleaved Spectrin alpha chain, non-erythrocytic 1 and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting.

Anti-SNTF (cleaved spectrin) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: SPTAN1, NEAS, SPTA2 UniProt Number: Q13813
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ABN2264-10UL
10 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, Porcine, HELISA, IHC, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberABN2264-10UL
DescriptionAnti-SNTF (cleaved spectrin)
Alternate Names
  • Spectrin alpha chain non-erythrocytic 1
  • Alpha-II spectrin
  • Fodrin alpha chain
  • Spectrin non-erythroid alpha subunit
  • Calpain-derived alpha-II spectrin N-terminal fragment
Background InformationSpectrin alpha chain, non-erythrocytic 1 (UniProt: Q13813; also known as Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha subunit) is encoded by the SPTAN1 (also known as NEAS, SPTA2) gene (Gene ID: 6709) in human. Non-erythroid Spectrin plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. Alpha II-spectrin is a structural protein abundant in neurons of the central nervous system and is cleaved into signature fragments by proteases involved in necrotic and apoptotic cell death. It is one of the primary targets cleaved by activated caspases during apoptosis. The full-length protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa. Alpha II- spectrin is involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. It has two calcium-binding regions (aa 2336-2347 and 2379-2390). Its mu-calpain cleavage site is localized at Tyr-Gly (aa 1176-1177). Phosphorylation of Tyrosine 1176 is shown to reduce sensitivity to cleavage by calpain in vitro. Plasma levels of calpain-cleaved Alpha II-Spectrin, N-terminal fragment (SNTF) serve as a useful marker for mild to severe traumatic brain injury and SNTF immunostaining can also reveal previously undetected traumatic brain injury. Mutations in SPTAN1 gene are known to cause early infantile epileptic encephalopathy that is characterized by seizures associated with hypsarrhythmia, profound mental retardation with lack of visual attention and speech development. (Ref.: Siman, R., et al. (2013). Front. Neurol. 4; Article 190; Johnson VE., et al (2016). Acta Neuropathol. 131(1); 115-135).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Applications
ApplicationAnti-SNTF (cleaved spectrin), Cat. No. ABN2264, is a highly specific rabbit polyclonal antibody that targets cleaved Spectrin alpha chain, non-erythrocytic 1 and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting.
Key Applications
  • ELISA
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected SNTF (cleaved spectrin) in swine and human brain sections (Johnson, V.E., et. al. (2016). Acta Neuropathol. 131(1):115-35).

Western Blotting Analysis: A representative lot detected SNTF (cleaved spectrin) in Western Blotting applications (Roberts-Lewis, J.M., et. al. (1994). J Neurosci. 14(6):3934-44).

Enzyme Immunoassay Analysis (ELISA): A representative lot detected SNTF (cleaved spectrin) in detection antibody for human serum samples (Siman, R., et. al. (2013). Front Neurol. 4:190).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to calpain-generated neoepitope in SNTF (CAQQEVY).
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects calpain-generated neoepitope in SNTF. It targets a neoepitope of 6 amino acids at the carboxyl-end of the stable calpain-derived alpha-spectrin.
IsotypeIgG
Species Reactivity
  • Mouse
  • Porcine
  • Human
Species Reactivity NoteHuman, Mouse, Porcine. Predicted to react with Rat and Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SPTAN1
  • NEAS
  • SPTA2
Purification MethodUnpurified
UniProt Number
Molecular Weight~160 kDa observed. Calculated molecular weight of full length protein is 284.54 kDa. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain extract incubated with calcium chloride to activate calpain.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Spectrin alpha chain, non-erythrocytic 1 in Mouse Brain extract incubated with calcium chloride to activate Calpain.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size10 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN2264-10UL 04054839499548