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487912 Anti-SNAP-25 Rabbit pAb

Anti-SNAP-25 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Synaptosomal Associated Protein-25

487912
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~25 kDa SNAP-25 protein in rat brain membranes.
Catalogue Number487912
Brand Family Calbiochem®
SynonymsAnti-Synaptosomal Associated Protein-25
References
ReferencesSollner, T., and Rothman, J.E. 1994. Trends Neurosci. 17, 344
Oyler, G.A., et al. 1989. J. Cell Biol. 109, 3039.
Product Information
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, pH 7.4, 1% BSA, 5% sucrose and 50 µg lyophilized control protein.
Positive controlSNAP-25-GST fusion protein or rat brain membranes
Preservative≤0.1% sodium azide (antibody only)
Applications
Application ReferencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609 9. Paraffin Sections Honer, W.G., et al. 1994. Acta Neuropathol. 87, 202. Young, C.E., et al. 1998. Cereb. Cortex 8, 261. ELISA and Immunoblotting Young, C.E., et al. 1998. Cereb. Cortex 8, 261.
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Immunoprecipitation
Application NotesImmunoblotting (1:1000, see application references)
Frozen Sections (see application references)
Paraffin Sections (see application references)
Immunoprecipitation (see comments)
ELISA (see application references)
Application CommentsFor a pre-adsorption control use 5 µg positive control protein for each µg of antibody. For immunoblotting use 20 ng positive control protein per lane (minigel). This antibody is also reported to work for immunoprecipitation. Antibody should be titrated for optimal use in individual systems.
Biological Information
Immunogena recombinant protein consisting of full-length rat SNAP-25 fused to GST
ImmunogenRat
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute antibody in 50 µl deionized H₂O to yield a final concentration of 1 mg/ml. Reconstituted antibody may be stored at 4°C for up to 2 weeks. For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of stock solution. Further dilutions should be made with buffer containing 1% BSA. Centrifuge all antibody preparations prior to use. Reconstitute positive control protein with 100 µl PBS. Following reconstitution of control, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
487912 0

Documentation

Anti-SNAP-25 Rabbit pAb Certificates of Analysis

TitleLot Number
487912

References

Reference overview
Sollner, T., and Rothman, J.E. 1994. Trends Neurosci. 17, 344
Oyler, G.A., et al. 1989. J. Cell Biol. 109, 3039.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-June-2010 JSW
SynonymsAnti-Synaptosomal Associated Protein-25
ApplicationImmunoblotting (1:1000, see application references)
Frozen Sections (see application references)
Paraffin Sections (see application references)
Immunoprecipitation (see comments)
ELISA (see application references)
DescriptionRabbit polyclonal antibody pre-adsorbed against immobilized GST, followed by protein A purification. Recognizes the ~25 kDa SNAP-25 protein. Supplied with a positive control consisting of lyophilized SNAP-25-GST fusion protein.
BackgroundSNAP-25 (synaptosome-associated protein of ~25 kDa) is an integral membrane protein localized to the plasma membrane of pre-synaptic axon terminals. As a component of the receptor for SNAPs (Soluble N-ethylmaleimide-sensitive factor [NSF] Attachment Proteins), SNAP-25 appears to play a critical role in the docking and fusion of pre-synaptic vesicles during exocytosis of neurotransmitters. This anti-SNAP-25 antibody was raised against a highly purified fusion protein of Schistosoma japonicum glutathione-S-transferase (GST) and rat SNAP-25.
HostRabbit
Immunogen speciesRat
Immunogena recombinant protein consisting of full-length rat SNAP-25 fused to GST
IsotypeIgG
SpeciesTorpedo sp. (Electric Ray), crayfish, mouse, rat
Positive controlSNAP-25-GST fusion protein or rat brain membranes
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, pH 7.4, 1% BSA, 5% sucrose and 50 µg lyophilized control protein.
Preservative≤0.1% sodium azide (antibody only)
CommentsFor a pre-adsorption control use 5 µg positive control protein for each µg of antibody. For immunoblotting use 20 ng positive control protein per lane (minigel). This antibody is also reported to work for immunoprecipitation. Antibody should be titrated for optimal use in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute antibody in 50 µl deionized H₂O to yield a final concentration of 1 mg/ml. Reconstituted antibody may be stored at 4°C for up to 2 weeks. For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of stock solution. Further dilutions should be made with buffer containing 1% BSA. Centrifuge all antibody preparations prior to use. Reconstitute positive control protein with 100 µl PBS. Following reconstitution of control, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesSollner, T., and Rothman, J.E. 1994. Trends Neurosci. 17, 344
Oyler, G.A., et al. 1989. J. Cell Biol. 109, 3039.
Application referencesFrozen Sections Honer, W.G., et al. 1993. Brain Res. 609 9. Paraffin Sections Honer, W.G., et al. 1994. Acta Neuropathol. 87, 202. Young, C.E., et al. 1998. Cereb. Cortex 8, 261. ELISA and Immunoblotting Young, C.E., et al. 1998. Cereb. Cortex 8, 261.