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ABC1428 Anti-RecQ1

Anti-RecQ1, Cat. No. ABC1428, is a rabbit polyclonal antibody that detects ATP-dependent DNA helicase Q1 and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-RecQ1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: RECQL, RECQ1, RECQL1 UniProt Number: P46063
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ABC1428
100 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, IHC, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABC1428
DescriptionAnti-RecQ1
Alternate Names
  • ATP-dependent DNA helicase Q1
  • RecQ-like type 1
  • DNA-dependent ATPase Q1
  • RecQ protein-like 1
Background InformationATP-dependent DNA helicase Q1 (UniProt: P46063; also known as EC:3.6.4.12, DNA helicase, RecQ-like type 1, RecQ1, DNA-dependent ATPase Q1, RecQ protein-like 1) is encoded by the RECQL (also known as RECQ1, RECQL1) gene (Gene ID: 5965) in human. RecQ helicases are a ubiquitous family of DNA unwinding enzymes involved in the maintenance of chromosome stability. They participate in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Five members of the RecQ family have been reported in human cells: BLM, RECQ1, RECQ4, RECQ5, and WRN. RecQ1 displays high expression in heart, lung, skeletal muscle and kidney. It is also highly expressed in tumor cells and although brain exhibits low level of EecQ1, its expression is dramatically increased in human glioblastoma. Depletion of RecQ1 affects proliferation of glioblastoma cells and causes an increase in the level of DNA damage. RecQ1 depleted tumor cells are shown to be more sensitive to anti-tumor agents, such as hydroxyurea and temozolomide.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing Tris-citrate-phosphate buffer (pH 7.8) with 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RecQ1, Cat. No. ABC1428, is a rabbit polyclonal antibody that detects ATP-dependent DNA helicase Q1 and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunofluorescence Analysis: A representative lot detected RecQ1 in T98G cells after treatment with anti-RECQ1 siRNA or control siRNAs (Mendoza-Maldonado, R., et. al. (2011). Mol Cancer. 10:83).

Western Blotting Analysis: A representative lot detected RecQ1 in Western Blotting applications (Thangavel, S., et. al. (2015). J Cell Biol. 208(5):545-62).

Immunohistochemistry Analysis: A representative lot detected RecQ1 in tissues are perilesional sections from multiform glioblastoma, colon carcinoma,lung cancer and thyroid cancer (Mendoza-Maldonado, R., et. al. (2011). Mol Cancer. 10:83).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human ATP-dependent DNA helicase Q1 (RecQ1).
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects ATP-dependent DNA helicase Q1 (RecQ1) in human cells. It targets an epitope within 16 amino acids from the C-terminal end.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RECQL
  • RECQ1
  • RECQL1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~75 kDa observed; 73.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in IMR90 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RecQ1 in 10 µg of IMR90 human fetal lung cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC1428 04054839328787