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MABN2510-100UL Anti-RNF213 Antibody, clone 1C9

Anti-RNF213, clone 1C9, Cat. No. MABN2510, is a mouse monoclonal antibody that detects E3 ubiquitin-protein ligase (RNF213) and is tested for use in ELISA, Immunocytochemistry, and Western Blotting.

MABN2510-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2510-100UL
DescriptionAnti-RNF213 Antibody, clone 1C9
Alternate Names
  • E3 ubiquitin-protein ligase RNF213
  • EC:2.3.2.27
  • ALK lymphoma oligomerization partner on chromosome 17
  • Mysterin
  • RING finger protein 213
  • RING-type E3 ubiquitin transferase RNF213
Background InformationE3 ubiquitin-protein ligase RNF213 (UniProt: Q63HN8; also known as EC:2.3.2.27, ALK lymphoma oligomerization partner on chromosome 17, Mysterin, RING finger protein 213, RING-type E3 ubiquitin transferase RNF213) is encoded by the RNF213 (also known as ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR) gene (Gene ID: 57674) in human. RNF213 is a widely expressed, homooligomeric cytosolic protein that is involved in the non-canonical Wnt signaling pathway in vascular development. It acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, which leads to the inhibition of the non-canonical Wnt signaling pathway and promotes vessel regression. Its activity is induced by pro-inflammatory cytokines. RNF213 contains a Zinc finger domain (aa 3997-4036), which is required for its ubiquitin-protein ligase activity. It also contains an AAA domain, which is associated with ATPase activity. Four isoforms of RNF213 have been described that are produced by alternative splicing. Mutations in RNF213 gene have been linked to Moyamoya disease 2 (MYMY2) disease, a progressive cerebral angiopathy that can lead to transient ischemic attacks and/or cerebral infarction, and rupture of the collateral vessels can cause intracranial hemorrhage.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-RNF213, clone 1C9, Cat. No. MABN2510, is a mouse monoclonal antibody that detects E3 ubiquitin-protein ligase (RNF213) and is tested for use in ELISA, Immunocytochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • ELISA
Application NotesWestern Blotting Analysis: A representative lot detected RNF213 in Western Blotting applications (Kotani, Y., et. al. (2017). Sci Rep. 7:44293).

ELISA Analysis: A representative lot detected RNF213 in Western Blotting applications (Kotani, Y., et. al. (2017). Sci Rep. 7:44293).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFLAG-tagged recombinant protein corresponding to the first 349 amino acids from the N-terminal region of human RNF213.
EpitopeN-terminal
Clone1C9
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1C9 is a mouse monoclonal antibody that detects human E3 ubiquitin-protein ligase (RNF213). It targets an epitope within the N-terminal domain.
IsotypeIgMκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RNF213
  • ALO17
  • C17orf27
  • KIAA1554
  • KIAA1618
  • MYSTR
Purification MethodLigatrap
UniProt Number
Molecular Weight591.41 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected RNF213 in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2510-100UL 04065265324342