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MABN2592-100UL Anti-RBP3 Antibody, clone mAb5

Anti-RBP3, clone mAb5, Cat. No. MABN2592, is a mouse monoclonal antibody that detects Retinol-binding protein 3 and is tested for use in Electron Microscopy, Immunoprecipitation, and Western Blotting.

MABN2592-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABN2592-100UL
DescriptionAnti-RBP3 Antibody, clone mAb5
Alternate Names
  • Retinol-binding protein 3
  • Interphotoreceptor retinoid-binding protein
  • IRBP
  • Interstitial retinol-binding protein
  • Protein 7S
Background InformationRetinol-binding protein 3 (UniProt: P12661; also known as Interphotoreceptor retinoid-binding protein, IRBP, Interstitial retinol-binding protein, RBP3, Protein 7S) is encoded by the RBP3 gene (Gene ID: 281443) in bovine. RBP3 is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 18-22) that are subsequently cleaved off to produce the mature form. It is a lipophilic glycoprotein that is secreted by rod and cone photoreceptors and plays a major role in photoreceptor homeostasis as well as retinoid and polyunsaturated fatty acid transport between the neural retina and retinal pigment epithelium. Its expression is observed early in retinal differentiation. RBP3 shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. It can bind many retinoids and polyunsaturated fatty acids and in addition to retinoid binding, it also plays a role in retinal development and homeostasis. RBP3 also displays thiol-dependent antioxidant activity that maintains the delicate redox balance in the normal retina. RBP3 dysregulation has been linked to retinal diseases. Mice lacking RBP3 exhibit severe early and progressive photoreceptor degeneration that is characterized by a reduction in both length and numbers of cone sheaths. In mammals four protein subunits of RBP3 have been described (M1-4), each consisting of about 300 amino acids. Clone mAb5 has been mapped to M2 subunit of RBP3. (Ref.: Sears, AE., et al. (2020) FASEB J. 34(10); 13918-13934; Zheng, S., et al. (2020). Front. Cell. Neurosci. 14; Article 577935).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-RBP3, clone mAb5, Cat. No. MABN2592, is a mouse monoclonal antibody that detects Retinol-binding protein 3 and is tested for use in Electron Microscopy, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Electron Microscopy
  • Immunoprecipitation
Application NotesTested applications

Electron Microscopy: A representative lot detected RBP3 in Electron Microscopy applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).

Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP3 in Immunoprecipitation applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).

Western Blotting Analysis: A representative lot detected RBP3 in Western Blotting applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenPurified Retinol-binding protein 3 (RBP3) from bovine retina.
EpitopeN-terminal half
ClonemAb5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone mAb5 is a mouse monoclonal antibody that detects Retinol-binding protein 3 (RBP3). It targets an epitope within the N-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Bovine
Species Reactivity NoteBovine. Predicted to react with Human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RBP3
Purification MethodProtein G purified
UniProt Number
Molecular Weight~135 kDa observed; 139.7 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from Bovine retinal tissue.

Western Blotting Analysis: A 1:500 dilution of this antibody RBP3 in lysate from Bovine retinal tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2592-100UL 04065269137511