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MABF1995-25UL Anti-PvRBP2b Antibody, clone 3E9

Anti-PvRBP2b, clone 3E9, Cat. No. MABF1995, is a mouse monoclonal antibody that detects Reticulocyte-binding protein 2 in Plasmodium vivax and has been tested for use in ELISA, Flow Cytometry, Inhibition/ Function Assay, and Western Blotting.

MABF1995-25UL
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF1995-25UL
DescriptionAnti-PvRBP2b Antibody, clone 3E9
Alternate Names
  • Reticulocyte-binding protein 2
Background InformationReticulocyte-binding protein 2 (UniProt: Q00799; also known as PvRBP2) is encoded by the RBP-2 (also known as RBP2) gene in Plasmodium species. PvRBP2b is an alpha-helical protein, comprising 10 alpha-helices and two very short antiparallel beta-sheets, each comprising two beta-strands. It plays a critical role in reticulocyte recognition and invasion by Plasmodium vivax. It is synthesized with a signal peptide (aa 1-21), a large extracellular domain (aa 22-2805), a short transmembrane domain (aa 2806-2826), and a cytoplasmic tail (aa 2827-2867). Plasmodium vivax is shown to preferentially invade reticulocytes that express high levels of transferrin receptor 1 (TfR1). PvRBP2b is expressed during the late-stage of Plasmodium vivax and its binding has been shown to directly correlate with the levels of TfR1 on reticulocyte surface. Treatment of reticulocytes with trypsin or chymotrypsin can cleave TfR1 from reticulocyte surface and prevent PvRBP2b binding. Clone 3E9 is shown to inhibit reticulocyte binding and block the entry of Plasmodium vivax into reticulocytes. This antibody binds to the side of the N-terminal domain of PvRBP2b to a region localized between helices alpha 4, 5 and 6. (Ref.: Gruszczyk, J., et al. (2018). Science 359(6371); 48-55; Gruszczyk, J., et al. (2018). Nature. 559(7712); 135-139).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
Applications
ApplicationAnti-PvRBP2b, clone 3E9, Cat. No. MABF1995, is a mouse monoclonal antibody that detects Reticulocyte-binding protein 2 in Plasmodium vivax and has been tested for use in ELISA, Flow Cytometry, Inhibition/ Function Assay, and Western Blotting.
Key Applications
  • ELISA
  • Flow Cytometry
  • Inhibits Activity/Function
  • Western Blotting
Application NotesInhibits Activity/Function Analysis: A representative lot inhibited PvRBP2b binding to reticulocytes and blocked the invasion of P. vivax into reticulocytes. (Gruszczyk, J., et. al. (2018). Science. 359(6371):48-55).

Flow Cytometry Analysis: A representative lot detected PvRBP2b in Flow Cytometry applications (Gruszczyk, J., et. al. (2018). Science. 359(6371):48-55).

Enzyme Immunoassay Analysis: A representative lot detected PvRBP2b in ELISA applications (Gruszczyk, J., et. al. (2018). Science. 359(6371):48-55).

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Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 1294 amino acids from the N-terminal half of Plasmodium vivax Reticulocyte-binding protein 2 (PvRBP2b).
Epitopeextracellular domain
Clone3E9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3E9 is a mouse monoclonal antibody that specifically detects Reticulocyte binding protein 2b (PvRBP2b) in Plasmodium vivax.
IsotypeIgG2aκ
Species Reactivity
  • Parasite
Species Reactivity NotePlasmodium vivax.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RBP-2
  • RBP2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~120-150 kDa observed; 331.44 kDa calculated (for full length 2pVRBP2b). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Plasmodium vivax under non-reducing conditions.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected purified Reticulocyte-binding protein from Plasmodium vivax (2PvRBP2b) under non-reducing conditions.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1995-25UL 04054839997235

Documentation

Anti-PvRBP2b Antibody, clone 3E9 SDS

Title

Safety Data Sheet (SDS) 

Anti-PvRBP2b Antibody, clone 3E9 Certificates of Analysis

TitleLot Number
Anti-PvRBP2b, clone 3E9 - 3238884 3238884

References

Reference overviewPub Med ID
Transferrin receptor 1 is a reticulocyte-specific receptor for Plasmodium vivax.
Gruszczyk, J; Kanjee, U; Chan, LJ; Menant, S; Malleret, B; Lim, NTY; Schmidt, CQ; Mok, YF; Lin, KM; Pearson, RD; Rangel, G; Smith, BJ; Call, MJ; Weekes, MP; Griffin, MDW; Murphy, JM; Abraham, J; Sriprawat, K; Menezes, MJ; Ferreira, MU; Russell, B; Renia, L; Duraisingh, MT; Tham, WH
Science  359  48-55  2018

Show Abstract 		29302006 29302006