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MABT1512-25UG Anti-Podoplanin Antibody, clone 8.1.1

This syrian hamster monoclonal Anti-Podoplanin, clone 8.1.1, Cat. No. MABT1512, is a syrian hamster monoclonal antibody that detects murine Podoplanin and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-Podoplanin Antibody, clone 8.1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Pdpn, Gp38 UniProt Number: Q62011
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MABT1512-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, ICC, IH(P), IP, WBSHmPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT1512-25UG
DescriptionAnti-Podoplanin Antibody, clone 8.1.1
Alternate Names
  • Glycoprotein 38
  • Gp38
  • OTS-8
  • PA2.26 antigen
  • Transmembrane glycoprotein E11
  • E11
Background InformationPodoplanin (UniProt: Q62011; also known as Glycoprotein 38, Gp38, OTS-8, PA2.26 antigen, T1-alpha, T1A, Transmembrane glycoprotein E11) I encoded by the Pdpn (also known as Gp38) gene (Gene ID: 14726) in murine species. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. Podoplanin is detected at high levels in lung and brain, at lower levels in kidney, stomach, liver, spleen and esophagus, and not detected in skin and small intestine. It is expressed in epithelial cells of choroid plexus, ependyma, glomerulus and alveolus, in mesothelial cells and in endothelia of lymphatic vessels. It is also expressed in stromal cells of peripheral lymphoid tissue and thymic epithelial cells and is detected in carcinoma cell lines and cultured fibroblasts. High expression of Podoplanin is observed in colon carcinomas. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Podoplanin levels are down-regulated by treatment with puromycin aminonucleoside. Mice lacking or with defective podoplanin die at birth of respiratory failure due to a low number of attenuated type I cells, narrow and irregular air spaces, and defective formation of alveolar saccules. Administration of PMab-1 antibody is shown to reduce lymphangiogenesis in the corneal suture and ear wound healing murine models. It suppresses thioglycollate-induced macrophages infiltration at the site of wound healing. (Ref.: Maruyama, Y et al (2014). Invest.Ophthal. Visual Sci. 55(8); 4813-4822).
References
Product Information
FormatPurified
PresentationPurified syrian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationThis syrian hamster monoclonal Anti-Podoplanin, clone 8.1.1, Cat. No. MABT1512, is a syrian hamster monoclonal antibody that detects murine Podoplanin and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in mouse lung tissue sections.

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).

Immunocytochemistry Analysis: A representative lot detected Podoplanin in Immunocytochemstry applications (Kamo, N., et. al. (2006). Am J Physiol Gastrointest Liver Physiol. 292(2):G526-34; Cohen, J.N., et. al. (2010). J Exp Med. 207(4):681-8; Ishii, T., et. al. (2010). Cell Tissue Res. 339(3):505-12).

Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Podoplanin in Immunoprecipitation applications (Farr, A., et. al. (1992). J Histochem Cytochem. 40(5):651-64).
Biological Information
ImmunogenMurine thymic stromal cell line TE-7.
Epitopeextracellular domain
Clone8.1.1
ConcentrationPlease refer to lot specific datasheet.
HostSyrian Hamster
SpecificityClone 8.1.1
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Pdpn
  • Gp38
Purification MethodProtein G purified
UniProt Number
Molecular Weight18.23 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse lung tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Podoplanin in mouse lung tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1512-25UG 04054839532023