Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS1158 Anti-PTPRT Antibody, clone 1F7

View Products on Sigmaaldrich.com
MABS1158
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1158
DescriptionAnti-PTPRT Antibody, clone 1F7
Alternate Names
  • Receptor-type tyrosine-protein phosphatase T
  • R-PTP-T
  • RPTP-rho
  • RPTPrho
  • PTPRT
Background InformationReceptor-type tyrosine-protein phosphatase T (EC 3.1.3.48; UniProt O14522; also known as R-PTP-T, Receptor protein tyrosine phosphatase, Receptor-type tyrosine-protein phosphatase rho, RPTP-rho, RPTPrho) is encoded by the PTPRT (also known as KIAA0283) gene (Gene ID 11122) in human. RPTPrho/PTPRT is a member of the R2B subfamily of receptor-type protein tyrosine phosphatases (R-PTPs). Known RPTPrho/PTPRT substrates include BCR (Tyr177), paxillin (Tyr188), and STAT3 (Tyr705). In addition, in vitro pull-downs and cell-free dephosphorylation assays also suggest cadherin and catenin family of cell adhesion molecules as physiological substrates of RPTPrho/PTPRT. PTPRT is the single most commonly mutated PTPR gene in all sequenced human cancers, with the highest PTPRT mutation frequency found in cutaneous melanoma. Among 16 tumor types examined, 37.9% of PTPRT mutations are found in the catalytic (PTPase) domain and 33.0% in the extracellular fibronectin type III-like (FN3) domain. In head and neck squamous cell carcinoma (HNSCC), 45.5% PTPRT mutations are located in the PTPase domain, leading to up-regulated STAT3 phosphorylation in HNSCC tumors.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PTPRT Antibody, clone 1F7 is an antibody against PTPRT for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 1.0 µg/mL of this antibody detected the full-length and cleaved forms of PTPRT in rat brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected the full-length and a cleaved form of PTPRT in brain and liver homogenates from wild-type, but not PTPRT-knockout mice (Courtesy of Dr. Zhenghe Wang, Case Western Reserve University, Cleveland, OH).
Western Blotting Analysis: 2.0 µg/mL of this antibody detected the full-length and a cleaved form of PTPRT in small intestine epithelium homogenate from wild-type mice (Courtesy of Dr. Zhenghe Wang, Case Western Reserve University, Cleveland, OH).
Western Blotting Analysis: A representative lot detected exogenously expressed human PTPRT using transiently transfected HNCC CAL-33 (squamous carcinomas of the tongue) cells expressing wild-type, PTPase domain mutatant (A1022E), or FN3-domain mutatant (P497T) PTPRT, as well as in transfected HNSCC PCI-52-SD1 cells stably expressing wild-type human PTPRT (Lui, V.W., et al. (2014). Proc. Natl. Acad. Sci. U.S.A. 111(3):1114-1119).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the PTPase domains 1/2 of human PTPRT.
EpitopePTPase domains 1/2
Clone1F7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1F7 reacts with the full-length PTPRT as well as cleaved/truncated PTPRT forms that contain the two phosphatase domains.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Rat
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PTPRT
  • KIAA0283
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~ 190/90 kDa observed. Due to glycosylation, the full-length target band (~190 kDa) appears larger than the calculated molecular weight (162.1 kDa). Cleaved/truncated forms between ~70 kDa to 120 kDa can also be detected. It is believed that PTPRT is proteolytically cleaved upon activation. The intracellular portion containing the two protein tyrosine phosphatase (PTPase) domains then translocates into nucleus, where it dephosphorylates STAT3 (Zhang, X., et al. (2007). Proc. Natl. Acad. Sci. U.S.A. 104(10):4060-4064).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected the full-length and a cleaved form of PTPRT in mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1158 04055977294835

Documentation

Anti-PTPRT Antibody, clone 1F7 SDS

Title

Safety Data Sheet (SDS) 

Anti-PTPRT Antibody, clone 1F7 Certificates of Analysis

TitleLot Number
Anti-PTPRT, clone 1F7 - 3275031 3275031
Anti-PTPRT, clone 1F7 - 3756543 3756543
Anti-PTPRT, clone 1F7 - 3887045 3887045
Anti-PTPRT, clone 1F7 -Q2611629 Q2611629

References

Reference overviewPub Med ID
Frequent mutation of receptor protein tyrosine phosphatases provides a mechanism for STAT3 hyperactivation in head and neck cancer.
Lui, VW; Peyser, ND; Ng, PK; Hritz, J; Zeng, Y; Lu, Y; Li, H; Wang, L; Gilbert, BR; General, IJ; Bahar, I; Ju, Z; Wang, Z; Pendleton, KP; Xiao, X; Du, Y; Vries, JK; Hammerman, PS; Garraway, LA; Mills, GB; Johnson, DE; Grandis, JR
Proceedings of the National Academy of Sciences of the United States of America  111  1114-9  2014

Show Abstract
24395800 24395800

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications