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MABE175 Anti-PML Isoform II Antibody, clone 1A8.1

Anti-PML Isoform II Antibody, clone 1A8.1 is an antibody against PML Isoform II for use in Western Blotting, Immunocytochemistry.

Anti-PML Isoform II Antibody, clone 1A8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: PML, MYL, PP8675, RNF71, TRIM19 UniProt Number: P29590-8
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MABE175
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE175
DescriptionAnti-PML Isoform II Antibody, clone 1A8.1
Alternate Names
  • PML-2
  • PML-II
  • Protein PML isoform II
  • Promyelocytic leukemia protein isoform II
  • RING finger protein 71 isoform II
  • TRIM19kappa
  • Tripartite motif-containing protein 19 isoform II
Background InformationProtein PML (UniProt P29590; also known as Promyelocytic leukemia protein, RING finger protein 71, Tripartite motif-containing protein 19) is encoded by the PML (also known as MYL, PP8675, RNF71, TRIM19) gene (Gene ID 5371) in human. The human PML gene/protein adopted its name when it was originally discovered as an RARα fusion protein caused by chromosomal translocation in acute promyelocytic leukemia (APL) patients. In normal cells, the PML protein interacts with multiple protein partners in the nucleus to form PML nuclear bodies (PML-NBs) that mediate a variety of cellular functions including senescence, apoptosis, protein degradation, telemere stasbility, and antiviral defense. At least 12 PML isoforms are generated by alternative splicings, including six nuclear isoforms (PML-I to PML-VI) and the cytoplasmic isoform PML-VII (a.k.a. PML-VIIB), other spliced isoforms wihtout exon 6-coding sequence (a.a. 467-552) are likewise expected to be cytoplasmic due to the abscence of nuclear localization signal (NLS) encoded by exon 6. All isoforms share the same N-terminal region (a.a. 1-419) containing the RBCC/tripartite motif and may have related functions due to their common functional RBCC/TRIM domain, while increasing evidences also suggest that each PML isoform possesses distinct functions mediated by its specific C-terminal sequence. Isoform PML-1 (a.k.a. PML-I, TRIM19alpha; UniProt P29590-1) and PML-2 (a.k.a. PML-II or TRIM19kappa; UniProt P29590-8) share the same N-terminal 570-a.a. sequence (encoded by exons 1 to 7a), however PML-1 derives its remaining C-terminal sequence (a.a. 571-882) from exons 8a & 9, while PML-II derives its (a.a. 571-829) from exon 7b with a different reading frame as that of PML-V.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PML Isoform II Antibody, clone 1A8.1 is an antibody against PML Isoform II for use in Western Blotting, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 40 µg/mL from a representative lot immunostained 10% neutral-buffered formalin-fixed HEK293 cells transfected with human PML isoform II by non-fluorescent immunocytochemistry (Courtesy of Professor Ygal Haupt, Peter MacCallum Cancer Centre, East Melbourne, Australia).
Immunocytochemistry Analysis: 10 µg/mL from a representative lot immunostained 4% paraformaldehyde-fixed HEK293 cells transfected with human PML isoform II by fluorescent immunocytochemistry (Courtesy of Professor Ygal Haupt, Peter MacCallum Cancer Centre, East Melbourne, Australia).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal sequence of human PML Isoform II.
EpitopeC-terminal region.
Clone1A8.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1A8.1 was raised against an C-terminal region sequence unique to PML isoform II (PML-2), but not present in the other 11 PML spliced isoforms reported by UniProt (P29590).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PML
  • MYL
  • PP8675
  • RNF71
  • TRIM19
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~110 kDa observed. 90.72 kDa calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from PML-2 transfected HEK293 cells.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected the exogenously expressed human PML-2 in 10 µg of lysate from transfected HEK293 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE175 04055977349474