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MABT1346-100UG Anti-P-Cadherin Antibody, clone CSTEM29

Anti-P-Cadherin, clone CSTEM29, Cat. No. MABT1346, is a mouse monoclonal antibody that detects P-Cadherin and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

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MABT1346-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABT1346-100UG
DescriptionAnti-P-Cadherin Antibody, clone CSTEM29
Alternate Names
  • Cadherin-3
  • Placental Cadherin
Background InformationCadherin-3 (UniProt: P22223; also known as Placental cadherin, P-cadherin) is encoded by the CHD3 (also known as CDHP) gene (Gene ID: 1001) in human. Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells. P-cadherin is a class I cadherin that is expressed in myoepithelial cells and is also expressed during late pregnancy and lactation, when luminal epithelial cells secrete high levels of a soluble fragment of P-cadherin in human milk. It is a single pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24) and a propeptide (aa 25-107 that are subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 108-654), a transmembrane domain (aa 655-677), and a cytoplasmic domain (aa 678-829). It has 5 cadherin domains. Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections and thereby imparting a strong curvature to the full-length ectodomain. P-cadherin dysfunction is strongly associated with tumorigenesis and confers the malignant phenotype in cancer cells. Its overexpression is strongly associated with poor prognosis in several solid tumors. Its high expression is reported to be predictive of shorter overall survival in glioblastoma patients. It is also detected in plasma membrane during progression of pancreatic intraepithelial ductal adenocarcinoma. (Ref.: Martins, EP., et al. (2021). Mol. Oncol. doi: 10.1002/1878-0261; Yu, W., et al. (2019). Front. Oncol. 9; 989; Siret, C., et al. (2018). Br. J. Cancer. 118; 546-557).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-P-Cadherin, clone CSTEM29, Cat. No. MABT1346, is a mouse monoclonal antibody that detects P-Cadherin and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Immunocytochemistry
  • ELISA
Application NotesTested Applications

ELISA Analysis: A representative lot detected P-Cadherin in ELISA applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Flow Cytometry Analysis: A representative lot detected P-Cadherin in Flow Cytometry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Immunocytochemistry Analysis: A representative lot detected P-Cadherin in Immunocytochemistry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant protein fragment corresponding to the extracellular domain of human P-Cadherin (Cadherin 3).
EpitopeExtracellular domain
CloneCSTEM29
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CSTEM3 is a mouse monoclonal antibody that detects P-Cadherin (Cadherin 3). It targets an epitope within the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHD3
Purification MethodProtein G purified
UniProt Number
Molecular Weight~80 kDa observed; 91.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant human P-Cadherin.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human P-Cadherin.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1346-100UG 04065268528983