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MABD76 Anti-Oct-4 Antibody, clone 9B7

Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF.

Anti-Oct-4 Antibody, clone 9B7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Transcription Factors Gene Symbol: POU5F1, OCT3, OCT4, OTF3 UniProt Number: Q01860
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MABD76
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, IHC, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABD76
DescriptionAnti-Oct-4 Antibody, clone 9B7
Alternate Names
  • POU domain, class 5, transcription factor 1
  • Octamer-binding protein 3
  • Oct-3
  • Octamer-binding protein 4
  • Oct-4
  • Octamer-binding transcription factor 3
  • OTF-3
Background InformationThe POU5F1 gene expresses the POU-domain transcription factor, Octamer-4 (Oct-4), which is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
References
Product Information
FormatPurified
Control
  • Mouse embryonic stem cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.
Biological Information
ImmunogenRecombinant protein corresponding to human Oct-4.
Clone9B7
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human based on immunogen design.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Gene Symbol
  • POU5F1
  • OCT3
  • OCT4
  • OTF3
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

SUBUNIT STRUCTURE: Interacts with UBE2I and ZSCAN10 By similarity. Interacts with PKM2. Interacts with WWP2.

SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern.

TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Molecular Weight~39 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABD76 04053252533129

Documentation

Anti-Oct-4 Antibody, clone 9B7 SDS

Title

Safety Data Sheet (SDS) 

Anti-Oct-4 Antibody, clone 9B7 Certificates of Analysis

TitleLot Number
Anti-Oct-4, clone 9B7 - 2420544 2420544
Anti-Oct-4, clone 9B7 - 2013424 2013424
Anti-Oct-4, clone 9B7 - 2161167 2161167
Anti-Oct-4, clone 9B7 - 2362000 2362000
Anti-Oct-4, clone 9B7 - 3251026 3251026
Anti-Oct-4, clone 9B7 - 3810498 3810498
Anti-Oct-4, clone 9B7 - NRG1932577 NRG1932577
Anti-Oct-4, clone 9B7 -2505493 2505493
Anti-Oct-4, clone 9B7 -2768112 2768112
Anti-Oct-4, clone 9B7 -2784649 2784649
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