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ST1552 Anti-NPC1 Mouse mAb (4H2)

Anti-NPC1 Mouse mAb (4H2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Niemann-Pick Disease C1

ST1552
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the NPC1 protein.
Catalogue NumberST1552
Brand Family Calbiochem®
SynonymsAnti-Niemann-Pick Disease C1
Application Data
Detection of human NPC1 by immunoblotting. Sample: Partial recombinant NPC1. Primary antibody: Anti-NPC1 Mouse mAb (4H2) (Cat. No. ST1552) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 151-250 of human NPC1, expressed as a GST fusion protein
Clone4H2
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1552 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
SynonymsAnti-Niemann-Pick Disease C1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human NPC1 by immunoblotting. Sample: Partial recombinant NPC1. Primary antibody: Anti-NPC1 Mouse mAb (4H2) (Cat. No. ST1552) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the NPC1 protein.
BackgroundNPC1 is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this protein cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over-accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 151-250 of human NPC1, expressed as a GST fusion protein
Clone4H2
IsotypeIgG2a
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review