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MAB5778-I-100UL Anti-NMDAR2B Antibody, C-term Antibody, clone 1C6.5C4

Anti-NMDAR2B, C-term, clone 1C6.5C4, Cat. No. MAB5778-I, is a mouse monoclonal antibody that detects NMDAR2B and is tested for use in Western Blotting.

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MAB5778-I-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMAB5778-I-100UL
DescriptionAnti-NMDAR2B Antibody, C-term Antibody, clone 1C6.5C4
Alternate Names
  • Glutamate receptor ionotropic NMDA 2B
  • GluN2B
  • Glutamate [NMDA] receptor subunit epsilon-2
  • N-methyl D-aspartate receptor subtype 2B
  • NR2B
Background InformationGlutamate receptor ionotropic, NMDA 2B (UniProt: Q00960; also known as GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B) is encoded by the Grin2b gene (Gene ID: 24410) in rat. NMDA receptor complexes are ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. They are involved in long-term potentiation and neuronal plasticity and regulate the number of nerve cells during development. However, over-stimulation NMDA receptors can lead to neuronal degeneration. NMDAR2B is a multi-pass membrane protein, which is synthesized with a signal peptide (aa 1-26), that is subsequently cleaved off to generate the mature form that contains two extracellular domains, three cytoplasmic domains, and four transmembrane domains. It is strongly expressed at birth and its levels increase until P6 to P20 and then begins to decline to the adult level. NMDAR2B is a component of the NMDA receptor complex that functions as heterotetrameric, ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. The channel activation requires binding of glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+. (Ref.: Laurie, DJ., et al. (1997). Mol. Brain Res. 51(1-2); 23-32).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-NMDAR2B, C-term, clone 1C6.5C4, Cat. No. MAB5778-I, is a mouse monoclonal antibody that detects NMDAR2B and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected NMDAR2B in Western Blotting applications (Laurie, D.J., et. al. (1997). Mol. Brain Res. 51(1-2):23-32).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 136 amino acids from the C-terminal half of Rat NMDAR2B.
EpitopeC-terminal half
Clone1C6.5C4
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1C6.5C4 is a mouse monoclonal antibody that detects NMDAR2B. It targets an epitope within 136 amino acids from the C-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Rabbit
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rabbit, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Grin2b
Purification MethodProtein G purified
UniProt Number
Molecular Weight~170 kDa observed; 166.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NMDAR2B in Rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB5778-I-100UL 04065266553734