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OP13L Anti-N-Myc Mouse mAb (NCM II 100)

Anti-N-Myc Mouse mAb (NCM II 100) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP13L
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes N-myc as well as its cleavage products in IMR5 cells. Myc is a labile protein. Fresh lysates in the presence of protease inhibitor cocktails are recommended.

Catalogue NumberOP13L
Brand Family Calbiochem®
References
ReferencesBrondyk, W.H. 1991. Oncogene 6, 1269.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Annu. Rev. Gen. 20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
Slamon, D.J., et al. 1986. Science 232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
Brodeur, G.M., et al. 1984. Science 224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
Kohl, N.E., et al. 1983. Cell 35, 603.
Schwab, M., et al. 1983. Nature 305, 245.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Positive controlIMR5 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone, Immunofluorescence Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoblotting Brondyk, W.H. 1991. Oncogene 6, 1269. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoprecipitation Brondyk, W.H. 1991. Oncogene 6, 1269.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesFrozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample, see application references)
Application CommentsIt is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human, N-Myc fusion protein, expressed in E. coli
ImmunogenHuman
CloneNCM II 100
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP13L 0

Documentation

Anti-N-Myc Mouse mAb (NCM II 100) SDS

Title

Safety Data Sheet (SDS) 

Anti-N-Myc Mouse mAb (NCM II 100) Certificates of Analysis

TitleLot Number
OP13L

References

Reference overview
Brondyk, W.H. 1991. Oncogene 6, 1269.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Annu. Rev. Gen. 20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
Slamon, D.J., et al. 1986. Science 232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
Brodeur, G.M., et al. 1984. Science 224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
Kohl, N.E., et al. 1983. Cell 35, 603.
Schwab, M., et al. 1983. Nature 305, 245.

Citations

Title
  • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-October-2007 RFH
    ApplicationFrozen Sections (1-5 µg/ml)
    Immunoblotting (1-5 µg/ml, see application references)
    Immunofluorescence (1-5 µg/ml, see application references)
    Immunoprecipitation (1-2 µg/sample, see application references)
    DescriptionPurified mouse monoclonal antibody derived by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc as well as its cleavage products.
    BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
    HostMouse
    Immunogen speciesHuman
    Immunogenrecombinant, human, N-Myc fusion protein, expressed in E. coli
    CloneNCM II 100
    IsotypeIgG₁
    Specieshuman, mouse
    Positive controlIMR5 cells
    FormLyophilized
    FormulationLyophilized from a volatile buffer, 100 µg BSA.
    PreservativeNone
    CommentsIt is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsReconstitute the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
    Toxicity Standard Handling
    ReferencesBrondyk, W.H. 1991. Oncogene 6, 1269.
    Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
    LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
    Cole, M.D., 1986. Annu. Rev. Gen. 20, 361.
    Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
    Slamon, D.J., et al. 1986. Science 232, 768.
    Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
    Brodeur, G.M., et al. 1984. Science 224, 1121.
    Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
    Kohl, N.E., et al. 1983. Cell 35, 603.
    Schwab, M., et al. 1983. Nature 305, 245.
    Citation
  • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
    		•
    Application referencesOriginal Clone, Immunofluorescence Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoblotting Brondyk, W.H. 1991. Oncogene 6, 1269. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoprecipitation Brondyk, W.H. 1991. Oncogene 6, 1269.