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ABN1728 Anti-MeCP2

Anti-MeCP2 Antibody, Cat. No. ABN1728, is a highly specific rabbit polyclonal antibody that targets MeCP2 and has been tested in Immunofluorescence and Western Blotting.

Anti-MeCP2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: MECP2, AS, MRXS13, MRXSL, PPMX, RTT, AUTSX3 UniProt Number: P51608
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ABN1728
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IFRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1728
DescriptionAnti-MeCP2
Alternate Names
  • Methyl-CpG-binding protein 2
  • MeCp-2 protein
  • MeCp2
Background InformationMethyl-CpG-binding protein 2 (UniProt P51608; also known as MeCp-2 protein, MeCp2) is encoded by the MECP2 (also known as AS, MRXS13, MRXSL, PPMX, RTT, AUTSX3) gene (Gene ID 4204) in human. Methyl-CpG-binding proteins that are involved in gene expression regulation by targeting methylated DNA residues in gene promoters via a conserved methyl-CpG-binding domain (MBD; a.a. 90-162 of human MeCp2). Upon binding methylated CpG sites on the DNA strands, transcriptional repression domain (TRD; a.a. 207-492) then mediates association with SIN3A to recruit histone deacetylases (HDAC). The TRD region also contains a nuclear localization signal (NLS) sequence (a.a. 255-271) and a nuclear receptor co-repressor 2/NCoR2-interacting site (a.a. 269-309). MeCp2 protein is found in all cells in the body, including the brain, where it is found in high concentrations in neurons and is associated with maturation of the central nervous system (CNS) and in forming synaptic contacts. In contrast to other MBD family members, MECP2 is X-linked and subject to X chromosome inactivation. MECP2 gene mutations are linked to Rett syndrome (RTT), a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing PBS with 0.03% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-MeCP2 Antibody, Cat. No. ABN1728, is a highly specific rabbit polyclonal antibody that targets MeCP2 and has been tested in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesImmunofluorescence Analysis: A 1:1000 dilution from a representative lot detected MeCp2 in mouse brain tissue sections (Courtesy of EnCor Biotechnology Inc.).
Biological Information
ImmunogenLinear peptide corresponding to human MeCp2 C-terminal end sequence.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody targets MeCp2 C-terminal end sequence conserved between spliced isoform A (isoform Beta) and isoform B (isoform Alpha).
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteMouse, Human. Predicted to react with Rhesus Macaque, Bovine, and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MECP2
  • AS
  • MRXS13
  • MRXSL
  • PPMX
  • RTT
  • AUTSX3
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~70 kDa observed. 52.44/52.31 kDa (human/mouse isoform A or Beta) and 53.32/53.58 kDa (human/mouse isoform B or Alpha) calculated. The larger-than-predicted band size is consistent with that reported in the literature (Jarrar, M.H., et al. (2003). J. Child Neurol. 18(10):675-682). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MeCp2 in 10 µg of mouse brain nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1728 04054839055096