Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

DR1106 Anti-MSH6 Mouse mAb (1F2)

This Anti-MSH6 Mouse mAb (1F2) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of MSH6.

Anti-MSH6 Mouse mAb (1F2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MutS Homolog 6

View Products on Sigmaaldrich.com
DR1106
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~140-150 kDa MSH6 protein in HeLa cells and human kidney tissue.
Catalogue NumberDR1106
Brand Family Calbiochem®
SynonymsAnti-MutS Homolog 6
Application Data
Detection of human MSH6 by immunoblotting. Sample: Whole tissue extract from human kidney. Primary antibody: Anti-MSH6 Mouse mAb (1F2) (Cat. No. DR1106) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human MSH6 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-MSH6 Mouse mAb (1F2) (Cat. No. DR1106) (10 µg/ml). Detection: fluorescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHuman kidney tissue, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corrresponding to amino acids of 931-1031 human MSH6, expressed as a GST fusion protein
Clone1F2
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1106 0

Documentation

Anti-MSH6 Mouse mAb (1F2) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-MutS Homolog 6
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human MSH6 by immunoblotting. Sample: Whole tissue extract from human kidney. Primary antibody: Anti-MSH6 Mouse mAb (1F2) (Cat. No. DR1106) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human MSH6 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-MSH6 Mouse mAb (1F2) (Cat. No. DR1106) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~140-150 kDa MSH6 protein.
BackgroundMSH6 combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer.
HostMouse
ImmunogenA recombinant polypeptide corrresponding to amino acids of 931-1031 human MSH6, expressed as a GST fusion protein
Clone1F2
IsotypeIgG₁
Specieshuman
Positive controlHuman kidney tissue, HeLa cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review