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IM72 Anti-MMP-9 (Ab-8) Mouse mAb (IA5)

Anti-MMP-9 (Ab-8) Mouse mAb (IA5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase, Anti-Gelatinase B

IM72
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP-9 in HUVEC and dexamethasone-treated HT1080 cells.
Catalogue NumberIM72
Brand Family Calbiochem®
SynonymsAnti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase, Anti-Gelatinase B
References
ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
Product Information
DeclarationNot available for sale in Japan.
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlConditioned medium from dexamethasone-treated HT-1080 or HUVEC cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Not Frozen Sections
Not Paraffin Sections
Application NotesFrozen Sections (not recommended)
Immunoblotting (2-4 µg/ml)
Immunofluorescence (see comments)
Paraffin Sections (not recommended)
Application CommentsThis antibody has also been shown to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogennative, human pro-MMP-9
ImmunogenHuman
CloneIA5
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IM72 0

Documentation

Anti-MMP-9 (Ab-8) Mouse mAb (IA5) SDS

Title

Safety Data Sheet (SDS) 

Anti-MMP-9 (Ab-8) Mouse mAb (IA5) Certificates of Analysis

TitleLot Number
IM72

References

Reference overview
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
SynonymsAnti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase, Anti-Gelatinase B
ApplicationFrozen Sections (not recommended)
Immunoblotting (2-4 µg/ml)
Immunofluorescence (see comments)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p3-X63-Ag8.653 mouse myeloma cells. Recognizes the ~92 kDa latent and the ~86 kDa active forms of MMP-9.
BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in angiogenesis, arthritis, periodontitis, and metastasis. Matrix metalloproteinase-9 (MMP-9) also known as gelatinase B, 92 kDa gelatinase/type IV collagenase (EC 3.4.24.35) has a broad range of substrate specificity for native collagens including types IV, V, VII, and X as well as gelatin, proteoglycans and elastin. MMP-9 is secreted as a 92 kDa proenzyme and can be activated in vitro by organomercurials (e.g., 4-aminophenylmercuric acetate, APMA), trypsin, and α-chymotrypsin and in vivo by cathepsin G and MMP-3 to the 86 kDa active form. The activity of MMP-9 can be inhibited by TIMPs in a 1:1 molar ratio. TIMP-1 is known to bind exclusively to pro-MMP-9 (Kd ~35 nM) whereas TIMP-2 binds to pro-MMP-2 with a Kd ~5 nM. MMP-9 has been measured in human plasma from normal individuals and has been shown to be elevated in sera of patients with hepatocellular carcinomas.
HostMouse
Immunogen speciesHuman
Immunogennative, human pro-MMP-9
CloneIA5
IsotypeIgG₁
Specieshuman
Positive controlConditioned medium from dexamethasone-treated HT-1080 or HUVEC cells
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody has also been shown to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.