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MAB1614 Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2

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MAB1614
100 µg  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, Ma, Sqd, Su, H, M, RELISA, ICC, IHC, IP, FUNC, RIA, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1614
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2
Background InformationKinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.
Product Information
FormatPurified
Control
  • Brain tissue
PresentationPurified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Affects Function
  • Radioimmunoassay
  • Western Blotting
Application NotesImmunohistochemistry:
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.

Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.

Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.

EIA/RIA:
A previous lot of this antibody was used in RIA.

Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.

Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenBovine brain kinesin.
Epitopea.a.420-445
CloneH2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the heavy chain of kinesin near the N-terminus.
IsotypeIgG2b
Species Reactivity
  • Chicken
  • Mammals
  • Squid
  • Sea Urchin
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KIF5B
  • UKHC
  • KINH
  • KNS1
  • KNS
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P33176 # Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).
SIZE: 963 amino acids; 109685 Da
SUBUNIT: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 (By similarity). Interacts with SYBU.
SUBCELLULAR LOCATION: Note=Uniformly distributed between soma and neurites in hippocampal neurons (By similarity).
DOMAIN: SwissProt: P33176 Composed of three structural domains: a large globular N- terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
SIMILARITY: Belongs to the kinesin-like protein family. Kinesin subfamily. & Contains 1 kinesin-motor domain.
Molecular Weight124 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB1614 04053252727566