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AB3270 Anti-K+-Cl- Cotransporter 4 Antibody

Anti-K+-Cl-Cotransporter 4 Antibody is an antibody against K+-Cl-Cotransporter 4 for use in ELISA & WB.

Anti-K+-Cl- Cotransporter 4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: SLC12A7, KCC4, DKFZP434F076 UniProt Number: Q9Y666
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AB3270
50 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3270
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-K+-Cl- Cotransporter 4 Antibody
Alternate Names
  • KCC4
Product Information
FormatAffinity Purified
PresentationAffinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5, containing 0.2% BSA and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-K+-Cl-Cotransporter 4 Antibody is an antibody against K+-Cl-Cotransporter 4 for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot: 1-10 μg/mL using ECL.

Approximate size on reduced westerns is 120-140kDa; membrane preparations and proteinase inhibitors are strongly recommended. See protocol under Technical Service as www.chemicon.com.



ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenA 16 amino acid peptide within the cytoplasmic, N-terminus of mouse KCC4.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes K+ Cl- Cotransporter-4 (KCC4). The immunogen shows no significant sequence homology with other KCCs or CCC.

SPECIES REACTIVITIES: The immunogen sequence is 81% homologous with human KCC4. Reactivity with other species has not been confirmed.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC12A7
  • KCC4
  • DKFZP434F076
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9Y666 # Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).
SIZE: 1083 amino acids; 119106 Da
SUBUNIT: Homomultimer and heteromultimer with other K-Cl cotransporters (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Detected in muscle, brain, lung, heart and kidney.
SIMILARITY: SwissProt: Q9Y666 ## Belongs to the SLC12A transporter family.
MISCELLANEOUS: Activated by N-ethylmaleimide (NEM). Inhibited by furosemide, DIDS and bumetanide. The inhibition is much stronger in the presence of 50 mM K(+) in the uptake medium. Inhibited by DIOA.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µg
Global Trade Item Number
Catalogue Number GTIN
AB3270 04053252589294