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MABF2795-25UL Anti-IL-9 Antibody, clone MH9A4

Anti-IL-9, clone MH9A4, Cat. No. MABF2795, is a mouse monoclonal antibody that detects Interleukin-9 and is tested for use in ELISA and Flow Cytometry.

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MABF2795-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABF2795-25UL
DescriptionAnti-IL-9 Antibody, clone MH9A4
Alternate Names
  • Interleukin-9
  • Cytokine P40
  • T-cell growth factor P40
Background InformationInterleukin-9 (UniProt: P15248; also known as IL-9, Cytokine P40, T-cell growth factor P40) is encoded by the IL9 gene (Gene ID: 3578) in human. IL-9 is a pleiotropic cytokine that plays a significant role in tolerance and inflammation. It is expressed by a variety of immune cells. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form. It supports the growth of mast cells, T cells, and several other cell types, including erythroid progenitors, fetal thymocytes, human megakaryoblastic leukemic cells, and myeloid precursor cells. It is also reported to supports IL-2 independent and IL-4 independent growth of helper T-cells. It also plays a role in promoting IgE production by B cells. IL-9 actions are mediated via its binding to IL-9R that results in the formation the IL-9R heterocomplex. Following its binding to the IL-9R , the SHC is phosphorylated, which then binds to Grb2 that subsequently activates SoS, a GTP exchange factor for Ras GTPases. This results in the activation of Ras GTPase that activates Raf kinase followed by the activation of MEK1/2 and ERK1/2. Although IL-9 is reported to activate the MAPK pathway in several lymphoid and hematopoietic cells, this activation is weaker compared to other cytokines. Elevated levels of IL-9 and IL-9R are observed in lungs of asthmatic subjects. (Ref.: Chakraborty, S., et al. (2019). Int. J. Mol. Sci. 20(9); 2113).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-IL-9, clone MH9A4, Cat. No. MABF2795, is a mouse monoclonal antibody that detects Interleukin-9 and is tested for use in ELISA and Flow Cytometry.
Key Applications
  • ELISA
  • Flow Cytometry
Application NotesTested Applications

Flow Cytometry Analysis: A representative lot detected IL-9 in Flow Cytometry applications (Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).

ELISA Analysis: A representative lot detected IL-9 in ELISA applications (Jenmalm, M.C., et al. (2001). Clin Exp Allergy.;31(10):1528-35; Faulkner, H., et al. (2002). J Infect Dis.;185(5):665-72; Bottcher, M.F., et al. (2003). Pediatr Allergy Immunol.;14(5):345-50; Holz, L.E., et al. (2005). J Inflamm (Lond).;2:3; Chang, H.C., et al. (2010). Nat Immunol.;11(6):527-34; Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenOvalbumin-conjugated recombinant human Interleukin-9.
EpitopeUnknown
CloneMH9A4
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone MH9A4 is a mouse monoclonal antibody that detects human Interleukin-9.
IsotypeIgG2b
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL9
Purification MethodProtein G purified
UniProt Number
Molecular Weight15.91 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with recombinant human IL-9.

ELISA Analysis:: Various dilution of this antibody detected recombinant human Interleukin-9 (IL-9).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2795-25UL 04065266908121