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ST1120 Anti-IDE/Insulysin Rabbit pAb

Anti-IDE/Insulysin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Insulin Degrading Enzyme, Anti-Insulysin

ST1120
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~110 kDa IDE protein in HT1080 cells.
Catalogue NumberST1120
Brand Family Calbiochem®
SynonymsAnti-Insulin Degrading Enzyme, Anti-Insulysin
Application Data
Lane 1: Detection of human IDE/insulysin by immunoblotting. Sample: Whole cell lysate (20 µg) from HT1080 cells. Primay antibody: Anti-IDE/Insulysin Rabbit pAb (Cat. No. ST1120) (0.5 µg/ml). Detection: chemiluminescence. Lane 2. Detection of human IDE/insulysin by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HT1080 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-IDE/Insulysin Rabbit pAb (Cat. No. ST1120) (5 µg). Immunoblotting conditions: same as lane 1.
References
ReferencesQiu, W.Q. and Folstein, M.F. 2006. Neurobiol. Aging 27, 190.
Song, E.H., et al. 2005. J. Biol. Chem. 280, 17701.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Positive controlHT1080 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (0.5 µg/ml)
Immunoprecipitation (5 µg/500 µg total protein)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids at the N-terminus of human IDE/insulysin
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1120 0

Documentation

Anti-IDE/Insulysin Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-IDE/Insulysin Rabbit pAb Certificates of Analysis

TitleLot Number
ST1120

References

Reference overview
Qiu, W.Q. and Folstein, M.F. 2006. Neurobiol. Aging 27, 190.
Song, E.H., et al. 2005. J. Biol. Chem. 280, 17701.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-October-2007 RFH
SynonymsAnti-Insulin Degrading Enzyme, Anti-Insulysin
ApplicationImmunoblotting (0.5 µg/ml)
Immunoprecipitation (5 µg/500 µg total protein)
Application Data
Lane 1: Detection of human IDE/insulysin by immunoblotting. Sample: Whole cell lysate (20 µg) from HT1080 cells. Primay antibody: Anti-IDE/Insulysin Rabbit pAb (Cat. No. ST1120) (0.5 µg/ml). Detection: chemiluminescence. Lane 2. Detection of human IDE/insulysin by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HT1080 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-IDE/Insulysin Rabbit pAb (Cat. No. ST1120) (5 µg). Immunoblotting conditions: same as lane 1.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~110 kDa IDE/insulysin protein.
BackgroundInsulysin or insulin degrading enzyme (IDE; accession no. P14735), also known as insulinase, is evolutionarily conserved, ubiquitously expressed, ~110 kDa zinc metalloprotease. It is located in the cytosol, on the cell surface, and may also be secreted. IDE is the major enzyme responsible for degrading insulin and is, thus, critical for glucose, lipid, and protein metabolism, as well as for cell growth and differentiation. IDE also degrades cerebral Aβ, a protein whose cleaved peptides contribute to the pathology of Alzheimer's disease. Homozygous deletion of the IDE gene in mice results in an increase in the level of Aβ as high as 65%, as well as an increase of 180% in serum insulin levels accompanied by impaired glucose tolerance, which is a hallmark of type 2 diabetes mellitus (DM2). Although hyperinsulinemia and glucose intolerance are the hallmark of DM2, etiology of the disease and the specific role of IDE in this process is not well understood. In human genetic studies, the IDE region of chromosome 10q has shown linkage to late-onset AD, DM2, and fasting plasma glucose level. In a recent study IDE was also identified as a cellular receptor for chickenpox virus (VZV) glycoprotein E, gE, which is involved in viral entry and cell-to-cell spread.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids at the N-terminus of human IDE/insulysin
IsotypeIgG
Specieshuman
Positive controlHT1080 cells
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesQiu, W.Q. and Folstein, M.F. 2006. Neurobiol. Aging 27, 190.
Song, E.H., et al. 2005. J. Biol. Chem. 280, 17701.