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CA1031 Anti-ICMT (170-279) Rabbit pAb

Anti-ICMT (170-279) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Isoprenylcysteine carboxyl methyltransferase

CA1031
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~32 kDa human ICMT protein in ICMT-transfected Sf9 insect cells.
Catalogue NumberCA1031
Brand Family Calbiochem®
SynonymsAnti-Isoprenylcysteine carboxyl methyltransferase
Application Data
Detection of human ICMT by immunoblotting. Samples: whole cell lysate (lanes 1-3) (30 µg, 10 µg, and 3 µg, respectively) containing full-length ICMT from transfected Sf9 cells, whole cell lysate from untransfected Sf9 cells (lane 4), whole cell lysate containing the 12.9 kDa immunogen (lanes 5-7) (0.01 µg, 0.03 µg, and 0.1 µg, respectively) from transfected Sf9 cells. Primary antibody: Anti-ICMT (170-279) Rabbit pAb (Cat. No. CA1031) (0.5 µg/ml). Detection: chemiluminescence.
References
ReferencesAnderson, J.L., et al. 2005. J.Biol. Chem. 280, 29454.
Winter-Vann, S.G., et al. 2005. PNAS USA 102, 4336.
Product Information
FormLiquid
FormulationIn 20 mM PBS.
Positive controlICMT-transfected Sf9 cells
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (0.5-1 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 170-279 of human ICMT, expressed in Sf9 cells
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CA1031 0

Documentation

References

Reference overview
Anderson, J.L., et al. 2005. J.Biol. Chem. 280, 29454.
Winter-Vann, S.G., et al. 2005. PNAS USA 102, 4336.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
SynonymsAnti-Isoprenylcysteine carboxyl methyltransferase
ApplicationImmunoblotting (0.5-1 µg/ml)
Application Data
Detection of human ICMT by immunoblotting. Samples: whole cell lysate (lanes 1-3) (30 µg, 10 µg, and 3 µg, respectively) containing full-length ICMT from transfected Sf9 cells, whole cell lysate from untransfected Sf9 cells (lane 4), whole cell lysate containing the 12.9 kDa immunogen (lanes 5-7) (0.01 µg, 0.03 µg, and 0.1 µg, respectively) from transfected Sf9 cells. Primary antibody: Anti-ICMT (170-279) Rabbit pAb (Cat. No. CA1031) (0.5 µg/ml). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~32 kDa ICMT protein.
BackgroundIsoprenylcysteine methyl transferase (ICMT) is an enzyme that catalyzes the transfer of methyl groups to the C-terminal prenylated cysteine in Ras family proteins and other related signaling proteins. This methylation is necessary for proper membrane targeting and transforming capability of Ras. Small molecule inhibition of ICMT results in mislocalization of Ras, imparied signaling through Ras pathways, and blocking of anchorage-independent growth in specific colon cancer cell lines.
HostRabbit
Immunogen speciesHuman
Immunogena recombinant protein consisting of amino acids 170-279 of human ICMT, expressed in Sf9 cells
IsotypeIgG
Specieshuman, mouse, rat
Positive controlICMT-transfected Sf9 cells
FormLiquid
FormulationIn 20 mM PBS.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Regulatory Review
ReferencesAnderson, J.L., et al. 2005. J.Biol. Chem. 280, 29454.
Winter-Vann, S.G., et al. 2005. PNAS USA 102, 4336.