Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS1165 Anti-HSD17B3 Antibody, clone 3E11.2

Detect HSD17B3 using this mouse monoclonal Anti-HSD17B3 Antibody, clone 3E11.2, Cat. No. MABS1165, validated for use in Western Blotting.

Anti-HSD17B3 Antibody, clone 3E11.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: HSD17B3, EDH17B3, SDR12C2 UniProt Number: P37058
View Products on Sigmaaldrich.com
MABS1165
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1165
DescriptionAnti-HSD17B3 Antibody, clone 3E11.2
Alternate Names
  • Testosterone 17-beta-dehydrogenase 3
  • 17-beta-HSD 3
  • 17-beta-hydroxysteroid dehydrogenase type 3
  • Short chain dehydrogenase/reductase family 12C member 2
  • Testicular 17-beta-hydroxysteroid dehydrogenase
Background InformationTestosterone 17-beta-dehydrogenase 3 (EC 1.1.1.64; UniProt P37058; also known as 17-beta-HSD 3, 17-beta-hydroxysteroid dehydrogenase type 3, Short chain dehydrogenase/reductase family 12C member 2, Testicular 17-beta-hydroxysteroid dehydrogenase) is encoded by the HSD17B3 (also known as EDH17B3, SDR12C2) gene (Gene ID 3293) in human. 17 -hydroxysteroid dehydrogenases (17 -HSDs) mediate the activation and deactivation of sex steroids involving redox reactions centered about the C17 area of the steroid backbone. 17 -HSD3 catalyzes the final step in the steroidogenesis of the potent androgen testosterone (T) by selectively reducing the C17 ketone of 4-dione, utilizing NADPH as a cofactor, in testis and prostate. Upregulated 17 -HSD3 mRNA level is found in cancerous prostate biopsies when compared to normal tissue, while 17 -HSD3 deficiency caused by HSD17B3 gene mutations results in 46,XY Disorders of Sex Development (46,XY DSD).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect HSD17B3 using this mouse monoclonal Anti-HSD17B3 Antibody, clone 3E11.2, Cat. No. MABS1165, validated for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human HSD17B3.
Clone3E11.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3E11.2 targets an epitope within the N-terminal half of mouse HSD17B3 and the two human spliced isoforms reported by UniProt (P70385 and P37058).
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HSD17B3
  • EDH17B3
  • SDR12C2
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~37 kDa observed. 34.52/29.11 kDa (human isoform 1/2) and 34.33 kDa (mouse) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse testis tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HSD17B3 in 10 µg of mouse testis tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1165 04054839056338