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MABE971 Anti-HNF-1-beta Antibody, clone 12A5.1

Anti-HNF-1-beta Antibody, clone 12A5.1 is an antibody against HNF-1-beta for use in Western Blotting and Immunohistochemistry.

Anti-HNF-1-beta Antibody, clone 12A5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HNF1B, TCF2 UniProt Number: P35680
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MABE971
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE971
DescriptionAnti-HNF-1-beta Antibody, clone 12A5.1
Alternate Names
  • Hepatocyte nuclear factor 1-beta
  • HNF-1B
  • HNF-1-beta
  • Homeoprotein LFB3
  • Transcription factor 2
  • TCF-2
  • Variant hepatic nuclear factor 1
  • vHNF1
Background InformationHepatocyte nuclear factor 1-beta (UniProt P35680; also known as HNF-1-beta, HNF-1B, Homeoprotein LFB3, TCF-2, Transcription factor 2, Variant hepatic nuclear factor 1, vHNF1) is encoded by the HNF1B (also known as TCF2, VHNF1) gene (Gene ID 6928) in human. HNF-1-beta is involved in transcriptional and functional regulation of the liver and biliary system, as well as of the kidneys, urogenital tract and pancreatic β-cells. HNF-1-beta deficiency due to HNF1B mutations is a well known cause of maturity-onset diabetes of the young 5 (MODY5), also referred to as RCAD (renal cysts and diabetes) due to the frequent co-occurrence of renal cysts and/or other inborn urogenital abnormalities. HNF-1-beta is expressed in the gall bladder, liver and intrahepatic bile ducts of mouse embryos and in liver of adult mice. HNF-1-beta is shown to co-regulate morphogenesis of the biliary system and HNF1B knockout mice suffer from severe neonatal cholestasis and jaundice due to the abnormally developed gall bladder and dysfunctional intrahepatic bile ducts.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-HNF-1-beta Antibody, clone 12A5.1 is an antibody against HNF-1-beta for use in Western Blotting and Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected HNF1B in human kidney and rat liver tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the Internal (N-terminal half) of human HNF-1-beta.
EpitopeInternal (N-terminal half)
Clone12A5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with isoform 1 (A) and isoform 3 (C). Reactivity toward isoform 2 (B) and isoform 4 has not been determined.
IsotypeIgG2aκ
Species Reactivity
  • Rat
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HNF1B
  • TCF2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~61 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HNF1B in 10 µg of rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE971 04055977294859

Documentation

Anti-HNF-1-beta Antibody, clone 12A5.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-HNF-1-beta Antibody, clone 12A5.1 Certificates of Analysis

TitleLot Number
Anti-HNF-1-beta, clone 12A5.1 - 3378580 3378580
Anti-HNF-1-beta, clone 12A5.1 - 3660306 3660306
Anti-HNF-1-beta, clone 12A5.1 - 3849490 3849490
Anti-HNF-1-beta, clone 12A5.1 - 3899552 3899552
Anti-HNF-1-beta, clone 12A5.1 - 3929232 3929232
Anti-HNF-1-beta, clone 12A5.1 - 4071249 4071249
Anti-HNF-1-beta, clone 12A5.1 - 4124280 4124280
Anti-HNF-1-beta, clone 12A5.1 - 4188110 4188110
Anti-HNF-1-beta, clone 12A5.1 -Q2611628 Q2611628

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications