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MAB3291 Anti-H-Ras Antibody, clone 7D7.2

This Anti-H-Ras Antibody, clone 7D7.2 is validated for use in ELISA, WB for the detection of H-Ras.

Anti-H-Ras Antibody, clone 7D7.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: HRAS, c-bas/has, H-Ras-1, RASH1, Ha-Ras, K-ras, N-ras, p21ras, c-H-ras, H-RasIDX, CTLO, HRAS1 UniProt Number: P01112
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MAB3291
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RELISA, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3291
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-H-Ras Antibody, clone 7D7.2
References
Product Information
FormatPurified
Presentation0.02M phosphate buffer, 0.25M NaCl, pH 7.6, 0.1% sodium azide
Quality LevelMQ100
Applications
ApplicationThis Anti-H-Ras Antibody, clone 7D7.2 is validated for use in ELISA, WB for the detection of H-Ras.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot

ELISA

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenFull-length recombinant human H-Ras - GST fusion protein
Clone7D7.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes H-Ras (the oncogene of Harvey murine sarcoma virus). Approximate molecular weight of 21 kDa.
IsotypeIgG2b
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HRAS
  • c-bas/has
  • H-Ras-1
  • RASH1
  • Ha-Ras
  • K-ras
  • N-ras
  • p21ras
  • c-H-ras
  • H-RasIDX
  • CTLO
  • HRAS1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01112 # Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
SIZE: 189 amino acids; 21298 Da
SUBUNIT: In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus.
PTM: Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
DISEASE: SwissProt: P01112 # Defects in HRAS are the cause of Costello syndrome [MIM:218040]; also known as faciocutaneoskeletal syndrome. Costello syndrome is a rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities. & Mutations which change positions 12, 13 or 61 activate the potential of c-ras to transform cultured cells and are implicated in a variety of human tumors. & Defects in HRAS are a cause of bladder cancer [MIM:109800]. & Defects in HRAS are also the cause of oral squamous cell carcinoma (OSCC).
SIMILARITY: SwissProt: P01112 ## Belongs to the small GTPase superfamily. Ras family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3291 04053252317897

Documentation

Anti-H-Ras Antibody, clone 7D7.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-H-Ras Antibody, clone 7D7.2 Certificates of Analysis

TitleLot Number
MOUSE ANTI-(H)-RAS 3031653
MOUSE ANTI-(H)-RAS - 2530118 2530118
MOUSE ANTI-(H)-RAS - 3766792 3766792
MOUSE ANTI-(H)-RAS - 3862673 3862673
MOUSE ANTI-(H)-RAS -2570209 2570209
MOUSE ANTI-(H)-RAS MONOCLONAL ANTIBODY - 2430506 2430506
MOUSE ANTI-(H)-RAS MONOCLONAL ANTIBODY - 2446759 2446759
MOUSE ANTI-(H)-RAS MONOCLONAL ANTIBODY - 2042771 2042771
MOUSE ANTI-(H)-RAS MONOCLONAL ANTIBODY - 2165835 2165835
MOUSE ANTI-(H)-RAS MONOCLONAL ANTIBODY - 2278489 2278489
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References

Reference overviewPub Med ID
Ubiquitination of K-Ras enhances activation and facilitates binding to select downstream effectors.
Sasaki, AT; Carracedo, A; Locasale, JW; Anastasiou, D; Takeuchi, K; Kahoud, ER; Haviv, S; Asara, JM; Pandolfi, PP; Cantley, LC
Science signaling  4  ra13  2011

Show Abstract 		21386094 21386094

Data Sheet

Title
MOUSE ANTI-(H)-RAS MONOCLONAL ANTIBODY

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