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368673 Anti-Grp78 (645-654) Rabbit pAb

Anti-Grp78 (645-654) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-BiP

368673
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~78 kDa Grp78 protein.
Catalogue Number368673
Brand Family Calbiochem®
SynonymsAnti-BiP
Application Data
Detection of mouse Grp78 by immunoblotting. Samples: Molecular weight marker (lane 1), Grp78 (BiP) recombinant protein (SPP-765) (lane 2), whole cell lysate from CHO-K1 cells (lane 3), and extract from mouse liver microsome (lane 4). Primary antibody: Anti-Grp78 (645-654) Rabbit pAb (Cat. No. 368673) (1:1000). Detection: chemiluminescence.
References
ReferencesTing, J., and Lee, A.S. 1989. DNA 7, 275.
Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
Warren, G. 1987. Nature 327, 17.
Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
Application CommentsThe immunogen sequence is identical to the corresponding sequence in mouse and hamster Gfp78, but cross-reactivity has not been tested. To reduce background by immunoblotting, we recommend blocking with 10% (w/v) skim milk powder in PBS, 0.05% Tween®-20 detergent. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide (EEDTSEKDEL) corresponding to amino acids 645-654 of rat Grp78, conjugated to KLH
ImmunogenRat
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
368673 0

Documentation

Anti-Grp78 (645-654) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Grp78 (645-654) Rabbit pAb Certificates of Analysis

TitleLot Number
368673

References

Reference overview
Ting, J., and Lee, A.S. 1989. DNA 7, 275.
Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
Warren, G. 1987. Nature 327, 17.
Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-May-2008 JSW
SynonymsAnti-BiP
ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
Application Data
Detection of mouse Grp78 by immunoblotting. Samples: Molecular weight marker (lane 1), Grp78 (BiP) recombinant protein (SPP-765) (lane 2), whole cell lysate from CHO-K1 cells (lane 3), and extract from mouse liver microsome (lane 4). Primary antibody: Anti-Grp78 (645-654) Rabbit pAb (Cat. No. 368673) (1:1000). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~78 kDa Grp78 protein.
BackgroundGrp78 is a resident, soluble protein in the ER that is involved in the proper assembly and folding of newly synthesized proteins. Grp78 contains a C-terminal KDEL sequence for retention in the ER
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide (EEDTSEKDEL) corresponding to amino acids 645-654 of rat Grp78, conjugated to KLH
IsotypeIgG
Specieshamster, mouse, rat
FormLiquid
FormulationIn PBS, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThe immunogen sequence is identical to the corresponding sequence in mouse and hamster Gfp78, but cross-reactivity has not been tested. To reduce background by immunoblotting, we recommend blocking with 10% (w/v) skim milk powder in PBS, 0.05% Tween®-20 detergent. Variables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesTing, J., and Lee, A.S. 1989. DNA 7, 275.
Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
Warren, G. 1987. Nature 327, 17.
Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.