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ABS2053-25UG Anti-Glutaredoxin-2 (Grx2)

Anti-Glutaredoxin-2 (Grx2), Cat. No. ABS2053, is a rabbit polyclonal antibody that detects Glutaredoxin-2, mitochondrial, and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Glutaredoxin-2 (Grx2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: GLRX2, GRX2, CGI-133 UniProt Number: Q9NS18
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ABS2053-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS2053-25UG
DescriptionAnti-Glutaredoxin-2 (Grx2)
Alternate Names
  • Glutaredoxin-2
  • mitochondrial
Background InformationGlutaredoxin-2, mitochondrial (UniProt: Q9NS18) is encoded by the GLRX2 (also known as GRX2, CGI-133) gene (Gene ID: 51022) in human. Glutaredoxin-2 is a glutathione-dependent oxidoreductase that is widely expressed and facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Its active form is a monomer and it can dimerize to an inactive form where homodimers are linked by one 2Fe-2S cluster. The 2Fe-2S may serve as a redox sensor and the presence of one-electron oxidants or reductants can lead to the loss of the 2Fe-2S cluster and subsequent monomerization and activation of the enzyme. Glutaredoxin-2 acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. It can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Glutaredoxin-2 is known to efficiently catalyze both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression of glutaredoxin-2 decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Glutaredoxin-2 (Grx2), Cat. No. ABS2053, is a rabbit polyclonal antibody that detects Glutaredoxin-2, mitochondrial, and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Glutaredoxin-2 (Grx2) in human cerebellum and human tonsil tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Glutaredoxin-2 .
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects mitochondrial isoform of glutatedoxin-2. It targets an epitope with in 16 amino acids from the C-terminal half.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chimpanzee, Baboon, Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Gene Symbol
  • GLRX2
  • GRX2
  • CGI-133
Purification MethodAffinity Purified
UniProt Number
Molecular Weight17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Glutaredoxin-2 (Grx2) in 10 µg of A549 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2053-25UG 04054839499272