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PC246 Anti-GluR1 Rabbit pAb

Anti-GluR1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glutamate Receptor I

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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~105-107 kDa GluR1 protein in D425 Med cells and hippocampal neurons. Also cross-reacts with a 50 kDa protein in rat brain extracts.
Catalogue NumberPC246
Brand Family Calbiochem®
SynonymsAnti-Glutamate Receptor I
References
ReferencesZuo, J. et al. 1997. Nature 388, 769.
McHugh, T.J., et al. 1996. Cell 87, 1339.
Tsien, J.Z., et al. 1996. Cell 87, 1327.
Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology 44, S7.
Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci. 17, 31.
Bliss, T.V.P. and Collingridge, G.L. 1993. Nature 361, 31.
Lomeli, H., et al. 1993. FEBS Lett. 315, 318.
Molnar, E. et al. 1993. Neuroscience 53, 307.
Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev. 16, 13.
Beal, M.F. 1992. FASEB J. 6, 3338.
Rothman, S.M. 1992. Ann. NY Acad. Sci. 648, 132.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlD425 Med cells, hippocampal neurons
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunofluorescence Cottrell, J.R. 2004. Neuron 44, 677.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesImmunoblotting 1-5 µg/ml)
Immunofluorescence (10-20 µg/ml, see application references)
Application CommentsAlso recognizes a smaller band at ~50 kDa using whole brain extracts. Immunofluorescence of dissociated cultured rat hippocampal neurons gave a punctate distribution, consistent with previous reports of GluR1 distribution. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (RTSDSRDHTRVDWKR) corresponding to amino acids 271-285 of rat GluR1 (numbered from the signal peptide)
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC246 0

Documentation

Anti-GluR1 Rabbit pAb Certificates of Analysis

TitleLot Number
PC246

References

Reference overview
Zuo, J. et al. 1997. Nature 388, 769.
McHugh, T.J., et al. 1996. Cell 87, 1339.
Tsien, J.Z., et al. 1996. Cell 87, 1327.
Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology 44, S7.
Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci. 17, 31.
Bliss, T.V.P. and Collingridge, G.L. 1993. Nature 361, 31.
Lomeli, H., et al. 1993. FEBS Lett. 315, 318.
Molnar, E. et al. 1993. Neuroscience 53, 307.
Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev. 16, 13.
Beal, M.F. 1992. FASEB J. 6, 3338.
Rothman, S.M. 1992. Ann. NY Acad. Sci. 648, 132.

Brochure

Title
Pathways and Biomarkers of Glutamatergic Synapse Flyer
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-January-2011 JSW
SynonymsAnti-Glutamate Receptor I
ApplicationImmunoblotting 1-5 µg/ml)
Immunofluorescence (10-20 µg/ml, see application references)
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~105-107 kDa GluR1 protein.
BackgroundGlutamate is the major excitatory neurotransmitter in the central nervous system. Precise regulation of glutamate levels are necessary as excess glutamate is toxic to neurons, presumably through receptor activation which leads to elevated intracellular Ca2+ levels. Multiple glutamate receptors have been identified in the mammalian brain, including the ligand-gated ion channels (ionotropic glutamate receptors) which are permeable to cations. These receptors have been classified into multiple subtypes based upon pharmacological and electrophysiological data, and includes the α-amino-3-hydroxy-5-methyl-4-isoxaxole propionate (AMPA)/kainate, and N-methyl-D-aspartate (NMDA) receptors. The ligand-gated glutamate receptors are multimeric heteromers composed of distinct subunits. Another subfamily of glutamate receptors includes eight G protein-coupled metabotropic glutamate receptors. The delta receptors are believed to represent another class of ionotropic glutamate receptors, although glutamate binding and ion channel activity remain to be demonstrated. Glutamate receptors likely play a key role in learning and memory, and it has been proposed that several neurodegenerative diseases may involve neural cell death caused by excessive activation of the glutamate receptors.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide (RTSDSRDHTRVDWKR) corresponding to amino acids 271-285 of rat GluR1 (numbered from the signal peptide)
IsotypeIgG
Speciesrat
Positive controlD425 Med cells, hippocampal neurons
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAlso recognizes a smaller band at ~50 kDa using whole brain extracts. Immunofluorescence of dissociated cultured rat hippocampal neurons gave a punctate distribution, consistent with previous reports of GluR1 distribution. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesZuo, J. et al. 1997. Nature 388, 769.
McHugh, T.J., et al. 1996. Cell 87, 1339.
Tsien, J.Z., et al. 1996. Cell 87, 1327.
Greenamyre, J.T. and Porter, R.H.P. 1994. Neurology 44, S7.
Hollmann, M. and Heinemann, S. 1994. Ann. Rev. Neurosci. 17, 31.
Bliss, T.V.P. and Collingridge, G.L. 1993. Nature 361, 31.
Lomeli, H., et al. 1993. FEBS Lett. 315, 318.
Molnar, E. et al. 1993. Neuroscience 53, 307.
Advokat, C. and Pellegrin, A.I. 1992. Neurosci. Biobehav. Rev. 16, 13.
Beal, M.F. 1992. FASEB J. 6, 3338.
Rothman, S.M. 1992. Ann. NY Acad. Sci. 648, 132.
Application referencesImmunofluorescence Cottrell, J.R. 2004. Neuron 44, 677.