Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABT100 Anti-Girdin Antibody, CT, clone 10E6.1

This Anti-Girdin, C-terminus Antibody, clone 10E6.1 is validated for use in Western Blotting, IHC(P), ICC, Immunoprecipitation for the detection of Girdin, C-terminus, clone 10E6.1.

Anti-Girdin Antibody, CT, clone 10E6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: CCDC88A, APE, GRDN UniProt Number: Q3V6T2
View Products on Sigmaaldrich.com
MABT100
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P), ICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT100
DescriptionAnti-Girdin Antibody, CT, clone 10E6.1
Alternate Names
  • Girdin
  • Akt phosphorylation enhancer
  • Coiled-coil domain-containing protein 88A
  • G alpha-interacting vesicle-associated protein
  • GIV
  • Girders of actin filament
  • Hook-related protein 1
  • HkRP1
Background InformationGirdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.
References
Product Information
FormatPurified
Control
  • Human brain tissue lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Girdin, C-terminus Antibody, clone 10E6.1 is validated for use in Western Blotting, IHC(P), ICC, Immunoprecipitation for the detection of Girdin, C-terminus, clone 10E6.1.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Girdin in epithelial cells of human prostate tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in COS-7 cell lysate.
Biological Information
ImmunogenRecombinant protein corresponding to human Girdin.
Clone10E6.1
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Gene Symbol
  • CCDC88A
  • APE
  • GRDN
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation. Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome.

SUBUNIT STRUCTURE: Interacts (via C-terminus) with DISC1; the interaction is direct. Interacts with AKT proteins; the interaction is inhibited in presence of DISC1. Homodimer. The non-phosphorylated form interacts with phosphatidylinositol 4-phosphate [PI4P] and weakly with phosphatidylinositol 3-phosphate [PI3P]. Interacts with microtubules. Interacts with actin through its C-terminal domain. Interacts with the C-terminus of AKT1/PKB.

SUBCELLULAR LOCATION: Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Note: Localizes to the cell membrane through interaction with phosphoinositides.

TISSUE SPECIFICITY: Expressed ubiquitously.

POST-TRANSLATIONAL MODIFICATION: Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration.

SEQUENCE SIMILARITIES: Belongs to the CCDC88 family.

SEQUENCE CAUTION: The sequence AAY14932.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAD97945.1 differs from that shown. Reason: Intron retention at the C-terminus. The sequence CAI46020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Molecular Weight~216 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human brain tissue lysate.

Wesetern Blot Analysis: A 1:5000 dilution of this antibody detected Girdin in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT100 04053252643422

Documentation

Anti-Girdin Antibody, CT, clone 10E6.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-Girdin Antibody, CT, clone 10E6.1 Certificates of Analysis

TitleLot Number
Anti-Girdin, C-terminus, clone 10E6.1 - 2153088 2153088
Anti-Girdin, C-terminus, clone 10E6.1 - 2328392 2328392
Anti-Girdin, C-terminus, clone 10E6.1 - 2366608 2366608
Anti-Girdin, C-terminus, clone 10E6.1 - 3288500 3288500
Anti-Girdin, C-terminus, clone 10E6.1 - 3521073 3521073
Anti-Girdin, C-terminus, clone 10E6.1 - 3538139 3538139
Anti-Girdin, C-terminus, clone 10E6.1 - 3715121 3715121
Anti-Girdin, C-terminus, clone 10E6.1 - Q1988385 Q1988385
Anti-Girdin, C-terminus, clone 10E6.1 -2496223 2496223
Anti-Girdin, C-terminus, clone 10E6.1 -2659648 2659648
Show more

Newsletters / Publications

Title
Research Focus - Volume 4 - 2012