Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB3402B Anti-GFAP Antibody, Biotin Conjugate

View Products on Sigmaaldrich.com
MAB3402B
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, R, Po, ChIHCMBiotinMonoclonal Antibody
Description
Catalogue NumberMAB3402B
DescriptionAnti-GFAP Antibody, Biotin Conjugate
Alternate Names
  • Glial fibrillary acidic protein
  • GFAP
Background InformationGlial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Product Information
FormatBiotin
Control
  • Mouse adult brain tissue
PresentationPurified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationAnti-GFAP Antibody, Biotin Conjugate detects level of GFAP & has been published & validated for use in IH.
Key Applications
  • Immunohistochemistry
Biological Information
ImmunogenPurified glial filament protein (Debus, E., et al. (1983) Differentiation. 25:193-203.)
HostMouse
IsotypeIgG1
Species Reactivity
  • Mouse
  • Human
  • Rat
  • Pig
  • Chicken
Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human, Rat, Porcine, and Chicken based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq].
Gene Symbol
  • GFAP
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

SIZE: 432 amino acids; 49880 Da

SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.

SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.

DISEASE: Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

SIMILARITY: Belongs to the intermediate filament family.
Molecular Weight50 kDa calculated
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in mouse adult brain tissue.

Immunohistochemistry Analysis: 1:100 dilution of this antibody detected GFAP in mouse adult brain tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
MAB3402B 04053252531422