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DR1045 Anti-GCLC Mouse mAb (3H1)

Anti-GCLC, mouse monoclonal, clone 3H1, recognizes the ~70 kDa GCLC protein in HeLa and A431 cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.

Anti-GCLC Mouse mAb (3H1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glutamate-Cysteine Ligase Catalytic Subunit

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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~70 kDa GCLC protein in HeLa and A431 cells.
Catalogue NumberDR1045
Brand Family Calbiochem®
SynonymsAnti-Glutamate-Cysteine Ligase Catalytic Subunit
Application Data
Detection of human GCLC by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-GCLC Mouse mAb (3H1) (Cat. No. DR1045) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GCLC by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GCLC Mouse mAb (3H1) (Cat. No. DR1045) (10 µg/ml). Detection: fluorescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlA431 cells, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 528-638 of human GCLC, expressed as a GST fusion protein
Clone3H1
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1045 0

Documentation

Anti-GCLC Mouse mAb (3H1) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-Glutamate-Cysteine Ligase Catalytic Subunit
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human GCLC by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-GCLC Mouse mAb (3H1) (Cat. No. DR1045) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GCLC by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GCLC Mouse mAb (3H1) (Cat. No. DR1045) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monolconal antibody. Recognizes the ~70 kDa GCLC protein.
BackgroundGlutamate-cysteine ligase, also known as γ-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Deficiency of γ-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 528-638 of human GCLC, expressed as a GST fusion protein
Clone3H1
IsotypeIgG₁
Specieshuman
Positive controlA431 cells, HeLa cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review