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05-1466 Anti-FOXA1 Antibody, clone 2F83

Use Anti-FOXA1 Antibody, clone 2F83 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXA1 also known as Forkhead box protein A1, hepatocyte nuclear factor 3 alpha.

Anti-FOXA1 Antibody, clone 2F83 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: HNF-3-alpha, HNF-3A, HNF3A, TCF3A UniProt Number: P55317
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05-1466
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue Number05-1466
DescriptionAnti-FOXA1 Antibody, clone 2F83
Alternate Names
  • Forkhead box protein A1
  • forkhead box A1
  • hepatocyte nuclear factor 3, alpha
Background InformationFOXA1 (HNF3A, Hepatocyte nuclear factor 3-alpha) is member of the Forkhead family of winged-helix transcription factors. It is known to be involved in the development and differentiation of several organs including liver, kidney, pancreas, lung, prostate and mammary gland (Lupien, 2008). High expression of FOXA1 is often seen in tumors arising from both the prostate and breast where it is believed to interact with either the androgen receptor or estrogen receptor α, in each of the tumor type respectively (Lupien, 2008). In the case of ER, FOXA1 has gained notice because of its interactions with the cis-regulatory regions in heterochromatin and because it enhances the interaction of ER with chromatin (Badve, 2007).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-FOXA1 Antibody, clone 2F83 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXA1 also known as Forkhead box protein A1, hepatocyte nuclear factor 3 alpha.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemisty:
Independent labs have verified this clone to work in paraffin-embedded IHC (IH(P)) of various mouse tissues including both embryonic and adult brain, adult digestive tract, adult respiratory tract, and adult urinary tract. Staining was performed using the antibody at a 1:2,000 dilution, but dilutions and conditions must be user optimized.
Biological Information
ImmunogenRecombinant human GST-FOXA1 protein encompassing amino acids 7-86.
EpitopeN-terminus
Clone2F83
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody detects human FOXA1 (HNF3α).
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman and Mouse. Other species have not been tested.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. [provided by RefSeq]
Gene Symbol
  • HNF-3-alpha
  • HNF-3A
  • HNF3A
  • TCF3A
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes.

SUBCELLULAR LOCATION: Nucleus.

SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain.
Molecular Weight49 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by western blot on HeLa nuclear extracts.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect FOXA1 in HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1466 04053252511929

Documentation

Anti-FOXA1 Antibody, clone 2F83 SDS

Title

Safety Data Sheet (SDS) 

Anti-FOXA1 Antibody, clone 2F83 Certificates of Analysis

TitleLot Number
Anti-FOXA1, clone 2F83 3072320
Anti-FOXA1, clone 2F83 2465169
Anti-FOXA1, clone 2F83 - 2410226 2410226
Anti-FOXA1, clone 2F83 - 2460942 2460942
Anti-FOXA1, clone 2F83 - 2038291 2038291
Anti-FOXA1, clone 2F83 - 3488918 3488918
Anti-FOXA1, clone 2F83 - 3559035 3559035
Anti-FOXA1, clone 2F83 - 3729445 3729445
Anti-FOXA1, clone 2F83 - 3932167 3932167
Anti-FOXA1, clone 2F83 - 4048674 4048674
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